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转录组分析两种甘薯品种的块根，揭示了块根发育的重要基因和调控途径。

Transcriptomic analysis of tuberous root in two sweet potato varieties reveals the important genes and regulatory pathways in tuberous root development.

机构信息

National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, 530004, People's Republic of China.

Guangxi South Subtropical Agricultural Science Research Institute, Chongzuo, 532406, People's Republic of China.

出版信息

BMC Genomics. 2022 Jun 27;23(1):473. doi: 10.1186/s12864-022-08670-x.

DOI:10.1186/s12864-022-08670-x
PMID:35761189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9235109/
Abstract

BACKGROUND

Tuberous root formation and development is a complex process in sweet potato, which is regulated by multiple genes and environmental factors. However, the regulatory mechanism of tuberous root development is unclear.

RESULTS

In this study, the transcriptome of fibrous roots (R0) and tuberous roots in three developmental stages (Rl, R2, R3) were analyzed in two sweet potato varieties, GJS-8 and XGH. A total of 22,914 and 24,446 differentially expressed genes (DEGs) were identified in GJS-8 and XGH respectively, 15,920 differential genes were shared by GJS-8 and XGH. KEGG pathway enrichment analysis showed that the DEGs shared by GJS-8 and XGH were mainly involved in "plant hormone signal transduction" "starch and sucrose metabolism" and "MAPK signal transduction". Trihelix transcription factor (Tai6.25300) was found to be closely related to tuberous root enlargement by the comprehensive analysis of these DEGs and weighted gene co-expression network analysis (WGCNA).

CONCLUSION

A hypothetical model of genetic regulatory network for tuberous root development of sweet potato is proposed, which emphasizes that some specific signal transduction pathways like "plant hormone signal transduction" "Casignal" "MAPK signal transduction" and metabolic processes including "starch and sucrose metabolism" and "cell cycle and cell wall metabolism" are related to tuberous root development in sweet potato. These results provide new insights into the molecular mechanism of tuberous root development in sweet potato.

摘要

背景

块根的形成和发育是甘薯中的一个复杂过程，受多个基因和环境因素调控。然而，块根发育的调控机制尚不清楚。

结果

本研究分析了两个甘薯品种 GJS-8 和 XGH 的纤维根（R0）和三个发育阶段（Rl、R2、R3）的块根的转录组。在 GJS-8 和 XGH 中分别鉴定出 22914 和 24446 个差异表达基因（DEGs），GJS-8 和 XGH 共有 15920 个差异基因。KEGG 通路富集分析表明，GJS-8 和 XGH 共有的 DEGs 主要参与“植物激素信号转导”“淀粉和蔗糖代谢”和“MAPK 信号转导”。通过对这些 DEGs 和加权基因共表达网络分析（WGCNA）的综合分析，发现三螺旋转录因子（Tai6.25300）与块根膨大密切相关。

结论

提出了一个甘薯块根发育的遗传调控网络的假设模型，强调了一些特定的信号转导途径，如“植物激素信号转导”“钙信号”“MAPK 信号转导”，以及代谢过程，包括“淀粉和蔗糖代谢”和“细胞周期和细胞壁代谢”，与甘薯块根发育有关。这些结果为甘薯块根发育的分子机制提供了新的见解。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5772/9235109/a01ee3b1caff/12864_2022_8670_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5772/9235109/83b76f85a33c/12864_2022_8670_Fig8_HTML.jpg
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