Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 06269, USA.
BMC Plant Biol. 2022 Mar 25;22(1):143. doi: 10.1186/s12870-022-03518-8.
Aronia is a group of deciduous fruiting shrubs, of the Rosaceae family, native to eastern North America. Interest in Aronia has increased because of the high levels of dietary antioxidants in Aronia fruits. Using Illumina RNA-seq transcriptome analysis, this study investigates the molecular mechanisms of polyphenol biosynthesis during Aronia fruit development. Six A. melanocarpa (diploid) accessions were collected at four fruit developmental stages. De novo assembly was performed with 341 million clean reads from 24 samples and assembled into 90,008 transcripts with an average length of 801 bp. The transcriptome had 96.1% complete according to Benchmarking Universal Single-Copy Orthologs (BUSCOs). The differentially expressed genes (DEGs) were identified in flavonoid biosynthetic and metabolic processes, pigment biosynthesis, carbohydrate metabolic processes, and polysaccharide metabolic processes based on significant Gene Ontology (GO) biological terms. The expression of ten anthocyanin biosynthetic genes showed significant up-regulation during fruit development according to the transcriptomic data, which was further confirmed using qRT-PCR expression analyses. Additionally, transcription factor genes were identified among the DEGs. Using a transient expression assay, we confirmed that AmMYB10 induces anthocyanin biosynthesis. The de novo transcriptome data provides a valuable resource for the understanding the molecular mechanisms of fruit anthocyanin biosynthesis in Aronia and species of the Rosaceae family.
Aronia 是蔷薇科的落叶结实灌木群，原产于北美东部。由于 Aronia 果实中含有高水平的膳食抗氧化剂，因此人们对其产生了浓厚的兴趣。本研究使用 Illumina RNA-seq 转录组分析，研究了 Aronia 果实发育过程中多酚生物合成的分子机制。本研究从六个 A. melanocarpa（二倍体）品种在四个果实发育阶段收集了 24 个样本，共 3.41 亿个清洁读数进行从头组装，生成了 90008 个转录本，平均长度为 801bp。根据基准通用单拷贝同源基因（BUSCOs），转录组的完整度为 96.1%。基于显著的基因本体论（GO）生物术语，在类黄酮生物合成和代谢过程、色素生物合成、碳水化合物代谢过程和多糖代谢过程中鉴定到差异表达基因（DEGs）。根据转录组数据，十个花青素生物合成基因在果实发育过程中表现出显著上调，qRT-PCR 表达分析进一步证实了这一点。此外，还在 DEGs 中鉴定到了转录因子基因。通过瞬时表达测定，我们证实了 AmMYB10 诱导了花青素的生物合成。从头转录组数据为理解 Aronia 和蔷薇科水果中果实花青素生物合成的分子机制提供了有价值的资源。
