Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou, China.
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, P.R. China.
Endocr Metab Immune Disord Drug Targets. 2023;23(5):692-701. doi: 10.2174/1871530322666220627151830.
Quercetin is a kind of flavonoid with important bioactivities, such as hypoglycemic, antioxidant, anti-inflammatory, and anti-allergic properties. Although it is unstable, it is worth exploring how to better exert its anti-allergic effect.
The current study aimed to elucidate the anti-allergic effect of quercetin liposomes on RBL-2H3 cells in vitro.
Quercetin liposomes were prepared to improve the anti-allergic activity of quercetin through a green thin-film dispersion method. We compared the anti-allergic effects of quercetin and quercetin liposomes in RBL-2H3 cells. The anti-allergic activity of the quercetin liposomes was evaluated by the level of β-hexosaminidase, histamine, Ca, IL-4, IL-8, and MCP-1.
The results showed that quercetin liposomes could significantly restrain the release of β-hexosaminidase and histamine, calcium influx, and the expression of inflammatory factors, whose effect is stronger than quercetin.
Collectively, our research suggests that the quercetin liposome can be used as a potential allergy antagonist.
槲皮素是一种具有重要生物活性的类黄酮，具有降血糖、抗氧化、抗炎和抗过敏等特性。尽管它不稳定，但值得探索如何更好地发挥其抗过敏作用。
本研究旨在阐明槲皮素脂质体体外对 RBL-2H3 细胞的抗过敏作用。
采用绿色薄膜分散法制备槲皮素脂质体，以提高槲皮素的抗过敏活性。我们比较了 RBL-2H3 细胞中槲皮素和槲皮素脂质体的抗过敏作用。通过β-己糖胺酶、组胺、Ca、IL-4、IL-8 和 MCP-1 的水平评估槲皮素脂质体的抗过敏活性。
结果表明，槲皮素脂质体可显著抑制β-己糖胺酶和组胺的释放、钙离子内流以及炎症因子的表达，其作用强于槲皮素。
综上所述，我们的研究表明槲皮素脂质体可用作潜在的过敏拮抗剂。
