Morimoto Yukiko, Yasuhara Tadashi, Sugimoto Akiko, Inoue Atsuko, Hide Izumi, Akiyama Mitoshi, Nakata Yoshihiro
Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
J Pharmacol Sci. 2003 Jul;92(3):291-5. doi: 10.1254/jphs.92.291.
Following prolonged exposure to some of the flavonoids with RBL-2H3 cells, secretion of hexosaminidase, a granule constituent, stimulated by an immunologic was enhanced. RBL-2H3 cells do not normally respond to polybasic secretagogues, but as reported here, they do so after prolonged exposure. Effect of flavonoids on secretion of hexosaminidase was also investigated. Of the thirteen flavonoids, quercetin and fisetin were the most potent inhibitors. A structure-activity study indicated that the position, number, and substitution of the hydroxy group of the B ring and saturation of the C2-C3 bond are important factors affecting flavonoid inhibition of secretary granules in RBL-2H3 cells.
在用RBL - 2H3细胞长期暴露于某些黄酮类化合物后，免疫刺激产生的颗粒成分己糖胺酶的分泌增强。RBL - 2H3细胞通常对多碱性促分泌剂无反应，但如本文报道，长期暴露后它们会产生反应。还研究了黄酮类化合物对己糖胺酶分泌的影响。在这13种黄酮类化合物中，槲皮素和漆黄素是最有效的抑制剂。结构 - 活性研究表明，B环羟基的位置、数量和取代以及C2 - C3键的饱和度是影响黄酮类化合物对RBL - 2H3细胞分泌颗粒抑制作用的重要因素。
