College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
Food Res Int. 2022 Jul;157:111281. doi: 10.1016/j.foodres.2022.111281. Epub 2022 Apr 22.
Atherosclerosis (AS) is the underlying condition in most cardiovascular diseases, which is blood vessel inflammation participated by many factors. Collagen hydrolysate from salmo salar skin (SCH) obtained in this study showed strong anti-inflammatory activity, protection of endothelial cell injury, antioxidant activity, and anti-platelet aggregation activity in vitro, exhibiting a great potential of attenuating AS. In this study, multifunctional peptides FAGPPGGDGQPGAK and IAGPAGPRGPSGPA, which mainly showed strong anti-inflammatory activity, were identified from SCH after separation of ultrafiltration and column chromatography. Moreover, SCH (contained anti-platelet peptides and anti-inflammatory peptides) was observed to inhibit arterial intima thickening and plaques formation in apolipoprotein E-deficient (ApoE) mice fed with high-fat diets without side effects, exhibiting a comparable effect with aspirin. SCH showed combined effect on regulating serum biomarkers of inflammation (IL-6 and TNF-α), endothelial injury (MCP-1), platelet activation (TXB and PF4) and oxidative stress (MDA and CAT). This research suggested SCH as a potential dietary supplement for the primary prevention of AS.
动脉粥样硬化（AS）是大多数心血管疾病的基础病变，是由多种因素参与的血管炎症。本研究中获得的鲑鱼皮胶原蛋白水解物（SCH）具有很强的抗炎活性、保护内皮细胞损伤、抗氧化活性和抗血小板聚集活性，在体外表现出减轻 AS 的巨大潜力。在这项研究中，从 SCH 中分离出超滤液和柱层析后，鉴定出主要表现出很强抗炎活性的多功能肽 FAGPPGGDGQPGAK 和 IAGPAGPRGPSGPA。此外，SCH（含有抗血小板肽和抗炎肽）在喂食高脂肪饮食的载脂蛋白 E 缺陷（ApoE）小鼠中观察到抑制动脉内膜增厚和斑块形成，没有副作用，表现出与阿司匹林相当的效果。SCH 对调节炎症的血清生物标志物（IL-6 和 TNF-α）、内皮损伤（MCP-1）、血小板活化（TXB 和 PF4）和氧化应激（MDA 和 CAT）具有综合作用。这项研究表明，SCH 可作为预防 AS 的潜在膳食补充剂。
