利用肾脏代谢组学分析研究蛋清衍生肽 QIGLF 的潜在降压机制。
Underlying antihypertensive mechanism of egg white-derived peptide QIGLF using renal metabolomics analysis.
机构信息
College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China; School of Food Science and Engineering, Hainan University, Haikou 570228, PR China.
College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China.
出版信息
Food Res Int. 2022 Jul;157:111457. doi: 10.1016/j.foodres.2022.111457. Epub 2022 Jun 6.
The kidney is an important target organ in the treatment of hypertension, but the effect of peptide QIGLF with antihypertensive activity on kidneys remains unknown. In the work, we aimed to further understand the hypotensive effects of QIGLF in spontaneously hypertensive rats (SHRs) using widely targeted metabolomics technology to investigate the kidney metabolic profiling variations. After four weeks of oral administration, the results showed different renal metabolomics profiles between QIGLF and model groups. Besides, a total of 10 potential biomarkers were identified, that is, 3-hydroxybutanoate, 20-hydroxyeicosatetraenoic acid, 19(S)-hydroxyeicosatetraenoic acid, 15-oxoETE, L-ornithine, malonate, uridine, uridine 5'-monophosphate, argininosuccinic acid, and N-carbamoyl-L-aspartate. These metabolites might exhibit antihypertensive activity of QIGLF by regulating synthesis and degradation of ketone bodies, arachidonic acid metabolism, pyrimidine metabolism, and arginine biosynthesis. These findings suggest that QIGLF might alleviate hypertension by inhibiting renal inflammation, promoting natriuresis, and regulating renal nitric oxide production.
肾脏是高血压治疗的重要靶器官，但具有降压活性的肽 QIGLF 对肾脏的影响尚不清楚。在这项工作中，我们旨在使用广泛靶向代谢组学技术进一步了解 QIGLF 在自发性高血压大鼠（SHR）中的降压作用，以研究肾脏代谢组学特征的变化。经过四周的口服给药，结果显示 QIGLF 组和模型组的肾脏代谢组学图谱存在差异。此外，共鉴定出 10 个潜在的生物标志物，即 3-羟基丁酸、20-羟二十碳四烯酸、19(S)-羟二十碳四烯酸、15-氧二十碳四烯酸、L-鸟氨酸、丙二酸盐、尿苷、尿苷 5'-单磷酸、精氨琥珀酸和 N-碳氨酰-L-天冬氨酸。这些代谢物可能通过调节酮体的合成和降解、花生四烯酸代谢、嘧啶代谢和精氨酸生物合成来发挥 QIGLF 的降压作用。这些发现表明，QIGLF 可能通过抑制肾脏炎症、促进排钠和调节肾脏一氧化氮生成来缓解高血压。
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