Department of Food Technology and Science, Federal University of Santa Maria (UFSM), Santa Maria RS 97105-900, Brazil.
Department of Bioscience, Federal University of São Paulo (UNIFESP), Santos, São Paulo CEP 11015-020, Brazil.
Food Res Int. 2022 Jul;157:111469. doi: 10.1016/j.foodres.2022.111469. Epub 2022 Jun 6.
This study aimed to investigate the impact of different microalgal matrices on the bioaccessibility and uptake by Caco-2 cells of carotenoids and chlorophylls. In this way, the microalgal ingredients/products (whole dry biomass [WDB], whole ultrasonicated paste [WUP], and liposoluble pigment emulsion [LPE]) obtained from Chlorella vulgaris and Arthrospira platensis were submitted to in vitro simulated digestion. Apical uptake of pigments in micelles generated during the simulated digestion by Caco-2 human intestinal cells was determined. The influence of simulated digestion on carotenoid and chlorophyll stability and bioaccessibility was assessed by HPLC-PDA-MS/MS and the carotenoids and chlorophylls' bioaccessibility and cellular uptake were shown to be boosted according to the matrix (LPE > WUP > WDB). Our findings showed that Chlorella vulgaris and Arthrospira platensis could be considered in formulations when carotenoids and chlorophylls are the target molecules in the ingredients/products.
本研究旨在探究不同微藻基质对类胡萝卜素和叶绿素经 Caco-2 细胞生物利用度和摄取的影响。为此，采用体外模拟消化法对小球藻和螺旋藻获得的微藻成分/产品（全干生物量 [WDB]、全超声处理糊剂 [WUP] 和脂溶性色素乳剂 [LPE]）进行处理。通过测定 Caco-2 人肠细胞对模拟消化过程中微胶束中生成的色素的顶侧摄取，评估模拟消化对类胡萝卜素和叶绿素稳定性及生物利用度的影响。结果表明，根据基质的不同（LPE＞WUP＞WDB），类胡萝卜素和叶绿素的生物利用度和细胞摄取均得到提高。本研究结果表明，在配方中当目标分子为类胡萝卜素和叶绿素时，可考虑使用小球藻和螺旋藻。
