Huang M, Wang X Q, Wang Z H, Zhou C N, Wu M
Sci Sin B. 1986 Nov;29(11):1186-91.
This is the first report on the diethylnitrosamine-induced malignant transformation of human epithelial cells derived from fetal kidney. The transformed cells have prolonged life span and aneuploid karyotypes. They also form anchorage independent colonies in soft agar. With additional one or two NDEA treatments, these cells could elicit poorly differentiated carcinomas in immunosuppressed new-born rats. This experiment indicates that the human fetal kidney cells seem to be a good material for studying the mechanisms of carcinogenesis of human epithelial cell.
这是关于二乙基亚硝胺诱导人胎儿肾来源的上皮细胞发生恶性转化的首篇报道。转化后的细胞具有延长的寿命和非整倍体核型。它们还能在软琼脂中形成不依赖贴壁的集落。经过额外一到两次二乙基亚硝胺处理后，这些细胞能在免疫抑制的新生大鼠中引发低分化癌。该实验表明，人胎儿肾细胞似乎是研究人上皮细胞癌变机制的良好材料。
