Huang M, Wang Z H, Wang X Q, Wu M
Sci Sin B. 1986 Nov;29(11):1192-200.
This report, for the first time, demonstrates that diethyl-nitrosamine (NDEA) and a new nitrosamine compound-N-1-methylacetonyl-N-3-methylbutyl-nitrosamine (MAMBNA), which are suspicious carcinogens of esophageal cancer (EC) in Linxian, Henan Province, are able to cause the malignant transformation (altered morphology, extended life span, anchorage independent growth, invasiveness, tumorigenicity, etc.) of normal diploid fibroblasts derived from human fetal lung. Besides, the preselection for clonogenic cells, which seem to be more readily to be transformed, in soft agar before carcinogen treatment, could be considered as one of the useful ways in establishment of a model system of malignant transformation of human fibroblasts in vitro.
本报告首次证明，河南省林县食管癌（EC）的可疑致癌物二乙基亚硝胺（NDEA）和一种新的亚硝胺化合物——N-1-甲基丙酮基-N-3-甲基丁基亚硝胺（MAMBNA），能够导致源自人胎儿肺的正常二倍体成纤维细胞发生恶性转化（形态改变、寿命延长、锚定非依赖性生长、侵袭性、致瘤性等）。此外，在致癌物处理前，在软琼脂中对似乎更容易发生转化的克隆形成细胞进行预选，可被视为建立人成纤维细胞体外恶性转化模型系统的有用方法之一。
