评估核苷酸/核苷类似物干预引物依赖性病毒 RNA 依赖性 RNA 聚合酶。
Assessment of nucleotide/nucleoside analog intervention in primer-dependent viral RNA-dependent RNA polymerases.
机构信息
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, No.44 Xiao Hong Shan, Wuhan, Hubei 430071, China.
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, No.44 Xiao Hong Shan, Wuhan, Hubei 430071, China.
出版信息
STAR Protoc. 2022 Jun 21;3(3):101468. doi: 10.1016/j.xpro.2022.101468. eCollection 2022 Sep 16.
Nucleotide/nucleoside analogs (NAs) are important compounds used in antiviral drug development. To understand the action mode of NA drugs, we present an enzymology protocol to initially evaluate the intervention mechanism of the NTP forms of NAs on a coronaviral RNA-dependent RNA polymerase (RdRP). We describe the preparation of SARS-CoV-2 RdRP proteins and RNA constructs, followed by a primer-dependent RdRP assay to assess NTP forms of NAs. Two representative NA drugs, sofosbuvir and remdesivir, are used for demonstration of this protocol. For complete details on the use and execution of this protocol, please refer to Wu et al. (2021).
核苷酸/核苷类似物（NAs）是用于抗病毒药物开发的重要化合物。为了了解 NA 药物的作用模式，我们提出了一种酶学方案，最初评估 NAs 的 NTP 形式对冠状病毒 RNA 依赖性 RNA 聚合酶（RdRP）的干预机制。我们描述了 SARS-CoV-2 RdRP 蛋白和 RNA 构建体的制备，然后进行引物依赖性 RdRP 测定，以评估 NAs 的 NTP 形式。两种代表性的 NA 药物，索非布韦和瑞德西韦，用于演示该方案。有关该方案使用和执行的完整详细信息，请参阅 Wu 等人。（2021 年）。
Zotero 插件