血清外泌体 microRNA-370-3p 和 microRNA-196a-5p 是肝细胞癌诊断和预后的潜在生物标志物。

Serum exosomal microRNA-370-3p and microRNA-196a-5p are potential biomarkers for the diagnosis and prognosis of hepatocellular carcinoma.

机构信息

Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding City, Hebei Province 071000, People's Republic of China.

出版信息

Folia Histochem Cytobiol. 2022;60(3):215-225. doi: 10.5603/FHC.a2022.0019. Epub 2022 Jun 28.

DOI:10.5603/FHC.a2022.0019

PMID:35762276

Abstract

INTRODUCTION

Evidence has shown that some microRNAs (miRNAs) play a role in tumorigenesis of hepatocellular carcinoma (HCC). Herein, we aimed to evaluate the diagnostic and prognostic values of serum exosomal miR-370-3p and miR-196a-5p in patients with HCC.

MATERIAL AND METHODS

Serum exosomes in 90 HCC patients were extracted and identified. Serum exosomal miR-370-3p and miR-196a-5p expression in HCC patients were detected. The diagnostic value of miR-370-3p and miR-196a- 5p, relationship between miR-370-3p and miR-196a-5p expression and clinicopathological features and prognosis of patients with HCC were analyzed. Relationship between miR-370-3p and miR-196a-5p expression and liver function indices such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) in HCC patients were analyzed. The effects of miR-370-3p and miR-196a-5p on Huh-7 HCC cells' proliferation, invasion and migration were determined.

RESULTS

Lower expression of miR-370-3p and higher expression of miR-196a-5p were found in serum exosomes of HCC patients. Serum exosomal miR-370-3p and miR-196a-5p were associated with tumor size, tumor grade and TNM stage as well as prognosis and liver function indices of HCC patients. Overexpressed miR-370-3p or silenced miR-196a-5p suppressed proliferation, invasion and migration of Huh-7 HCC cells.

CONCLUSIONS

We suggest that miR-370-3p/miR-196a-5p in serum exosomes of HCC patients could be potential biomarkers for the diagnosis and prognosis of HCC.

摘要

简介

有证据表明，一些 microRNAs（miRNAs）在肝细胞癌（HCC）的发生中起作用。在此，我们旨在评估血清外泌体 miR-370-3p 和 miR-196a-5p 在 HCC 患者中的诊断和预后价值。

材料与方法

提取并鉴定 90 例 HCC 患者的血清外泌体。检测 HCC 患者血清外泌体 miR-370-3p 和 miR-196a-5p 的表达。分析 miR-370-3p 和 miR-196a-5p 的诊断价值，miR-370-3p 和 miR-196a-5p 表达与 HCC 患者临床病理特征和预后的关系，以及 miR-370-3p 和 miR-196a-5p 表达与 HCC 患者丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）和总胆红素（TBIL）等肝功能指标的关系。测定 miR-370-3p 和 miR-196a-5p 对 Huh-7 HCC 细胞增殖、侵袭和迁移的影响。

结果

发现 HCC 患者血清外泌体中 miR-370-3p 表达降低，miR-196a-5p 表达升高。血清外泌体 miR-370-3p 和 miR-196a-5p 与 HCC 患者的肿瘤大小、肿瘤分级和 TNM 分期以及预后和肝功能指标有关。过表达 miR-370-3p 或沉默 miR-196a-5p 抑制 Huh-7 HCC 细胞的增殖、侵袭和迁移。