Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra Precision Optical Manufacturing, Fudan University, Shanghai, China.
Key Laboratory of Laser Device Technology, China North Industries Group Corporation Limited, Southwest Institute of Technical Physics, Chengdu, China.
J Biophotonics. 2022 Oct;15(10):e202200151. doi: 10.1002/jbio.202200151. Epub 2022 Jul 9.
The measurement of cardiac troponin-I (cTnI) is widely used for diagnosing acute myocardial infarction (AMI) diseases because of its myocardial specificity. Packaged microbubble resonators with thin wall are utilized for label-free and specific detection of cTnI based on whispering gallery mode (WGM). This packaged structure can provide a good protection for the biosensor, improve the anti-interference ability of the sensor and reduce the system noise. The theoretical detection limit of the biosensors for cTnI in phosphate buffer saline (PBS) is 0.4 ag mL (0.02 a ). Furthermore, we demonstrated that the biosensors can be used to detect cTnI molecules in simulated serum and the theoretical detection limit is also 0.4 ag mL (0.02 a ). These results are much far below the clinical cut-off value and show a huge application potential for the detection of cardiac biomarkers of AMI.
心肌肌钙蛋白 I（cTnI）的测量因其心肌特异性而被广泛用于诊断急性心肌梗死（AMI）疾病。基于声回廊模式（WGM），采用薄壁封装微泡谐振器实现了对 cTnI 的无标记和特异性检测。这种封装结构可以为生物传感器提供良好的保护，提高传感器的抗干扰能力并降低系统噪声。生物传感器在磷酸盐缓冲盐水（PBS）中检测 cTnI 的理论检测限为 0.4 ag/ml（0.02 a）。此外，我们还证明，生物传感器可用于检测模拟血清中的 cTnI 分子，理论检测限也为 0.4 ag/ml（0.02 a）。这些结果远低于临床截止值，为 AMI 心脏生物标志物的检测展示了巨大的应用潜力。
