State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, China.
School of Marine Science, Ningbo University, 169 Qixing South Road, Ningbo, China.
Arch Microbiol. 2022 Jun 28;204(7):436. doi: 10.1007/s00203-022-03053-y.
In recent decades, the prevalence of hyperuricemia has increased, and dietary fructose is an important risk factor for the development of this disease. This study investigated and compared the effects of Sphacelotheca reiliana polysaccharides and Phoenix dactylifera monosaccharides on a series of physiological and biochemical indicators and on the metagenomes and serum metabolites in mice with hyperuricemia caused by a high-fructose diet. S. reiliana polysaccharides inhibited uric acid biosynthesis and promoted uric acid excretion, thereby alleviating the hyperuricemia phenotype. In addition, hyperuricemia was closely related to the gut microbiota. After treatment with S. reiliana polysaccharides, the abundances of Bacteroidetes and Proteobacteria in the mouse intestines were decreased, the expression of genes involved in glycolysis/gluconeogenesis metabolic pathways and purine metabolism was downregulated, and the dysfunction of the gut microbiota was alleviated. With regard to serum metabolism, the abundance of hippuric acid, uridine, kynurenic acid, propionic acid and arachidonoyl decreased, and the abundances of serum metabolites in inflammatory pathways involved in kidney injury and gout, such as bile acid metabolism, purine metabolism and tryptophan metabolism pathways, decreased. P. dactylifera monosaccharides aggravated hyperuricemia. This research provides a valuable reference for the development of sugar applications.
近几十年来，高尿酸血症的患病率不断增加，而膳食果糖是导致这种疾病发生的一个重要危险因素。本研究调查并比较了叶状地锦多糖和 1 种 1 般认为安全的食品原料（即，椰枣单糖）对高果糖饮食诱导的高尿酸血症小鼠一系列生理生化指标及宏基因组和血清代谢物的影响。叶状地锦多糖抑制尿酸合成并促进尿酸排泄，从而缓解高尿酸血症表型。此外，高尿酸血症与肠道微生物群密切相关。经叶状地锦多糖处理后，小鼠肠道中的拟杆菌门和变形菌门丰度降低，糖酵解/糖异生代谢途径和嘌呤代谢相关基因的表达下调，肠道微生物群功能失调得到缓解。关于血清代谢物，马尿酸、尿苷、犬尿氨酸、丙酸和花生四烯酸的丰度降低，涉及肾脏损伤和痛风的炎症途径的血清代谢物（如胆汁酸代谢、嘌呤代谢和色氨酸代谢途径）的丰度降低。椰枣单糖加重了高尿酸血症。本研究为糖的应用开发提供了有价值的参考。
