Jiangsu Key Laboratory for Organic Solid Waste Utilization, Educational Ministry Engineering Center of Resource-saving Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, PR China.
Laboratory for Microbial Resources, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, PR China.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005443.
Two Gram-stain-positive, non-motile, strictly aerobic, yellow-coloured, rod-shaped bacterial strains, designated LR1S40 and M4N3S171, were isolated from rhizosphere and bulk saline soil of collected in Inner Mongolia, China. Phylogenetic trees based on 16S rRNA gene and whole genome sequences showed that the two strains clustered tightly with strains of the genus . Strains LR1S40 and M4N3S171 had 95.5% 16S rRNA gene similarity to each other, and strain LR1S40 had 98.8, 98.7, 97.4 and <97.0% similarity to RN3S43, LNNU 22110, YIM 93306 and all other current type strains, while strain M4N3S171 had 98.6 and <97.0% similarity to YIM 93306, and all other current type strains, respectively. The average nucleotide identity based on blast (ANIb) and digital DNA-DNA hybridization (dDDH) values of LR1S40 and M4N3S171 with each other and to the other type strains of were well below the threshold values (95% for ANIb, 70% for dDDH) for differentiating a species. Diphosphatidylglycerol and phosphatidylglycerol were the major polar lipids in both strains. The predominant menaquinone in both strains was both MK-8. The genome of strain LR1S40 consisted of a 3557440 bp circular chromosome, with a G+C content of 71.1 mol%, while the genome of strain M4N3S171 consisted of 4270413 bp, with a G+C content of 67.6 mol%. The phylogenetic, physiological and phenotypic characteristics allowed discrimination of the two strains from their relatives. The names sp. nov. [type strain LR1S40 (=CGMCC 1.19028=KCTC 49726)] and sp. nov. [type strain M4N3S171 (=CGMCC 1. 19142=KCTC 49727)] are therefore proposed. During the publication of , (type strain T3246-1), which was selected as the reference strain for the identification of , was reclassified as . The two phylogenetic trees showed that HY164 tightly clustered with F300, and had the highest 16S rRNA gene similarity (99.8%) to F300. Based on the phylogenetic analysis and the publication record, should be reclassified as comb. nov.
两株革兰氏阳性、非运动性、严格需氧、黄色、杆状细菌菌株，分别命名为 LR1S40 和 M4N3S171，从中国内蒙古采集的根际和大量盐土中分离得到。基于 16S rRNA 基因和全基因组序列的系统发育树显示，这两株菌与 属的菌株紧密聚类。菌株 LR1S40 和 M4N3S171 的 16S rRNA 基因相似度为 95.5%，而菌株 LR1S40 与 RN3S43、LNNU 22110、YIM 93306 和所有其他当前模式菌株的相似度分别为 98.8%、98.7%、97.4%和 <97.0%，而菌株 M4N3S171 与 YIM 93306 和所有其他当前模式菌株的相似度分别为 98.6%和 <97.0%。LR1S40 和 M4N3S171 之间的平均核苷酸身份基于 blast（ANIb）和数字 DNA-DNA 杂交（dDDH）值与其他 属的模式菌株分别为 <95%和 <70%，低于区分种的阈值。二磷脂酰甘油和磷脂酰甘油是两种菌株的主要极性脂。两种菌株的主要类异戊二烯醌都是 MK-8。菌株 LR1S40 的基因组由一个 3557440 bp 的圆形染色体组成，GC 含量为 71.1 mol%，而菌株 M4N3S171 的基因组由 4270413 bp 组成，GC 含量为 67.6 mol%。基于系统发育、生理和表型特征，可将这两株菌与亲缘菌株区分开来。因此，提议将这两个菌株命名为. sp. nov. [模式菌株 LR1S40（=CGMCC 1.19028=KCTC 49726）]和. sp. nov. [模式菌株 M4N3S171（=CGMCC 1.19142=KCTC 49727）]。在发表期间，作为鉴定. 参考菌株的. T3246-1 被重新分类为. HY164 与. F300 紧密聚类，与. F300 的 16S rRNA 基因相似度最高（99.8%）。基于系统发育分析和发表记录，应将. 重新分类为. comb. nov.。
