Department of Environment Engineering, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, PR China.
Jiangsu Key Lab for Organic Solid Waste Utilization, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Curr Microbiol. 2021 Aug;78(8):3285-3291. doi: 10.1007/s00284-021-02579-6. Epub 2021 Jun 23.
A Gram-positive, strictly aerobic, ivory-colored, rod-shaped bacterial strain, designated RN3S43, was isolated from saline-alkaline soil, in Tumd Right Banner, Inner Mongolia, China. Strain RN3S43 grew at 10-40 °C (optimum 30 °C), pH 6.0-10.0 (optimum pH 9.0), and 0-12.5% NaCl (optimum 2-4%). It was positive to oxidase, catalase, urease, and nitrate reductase. The methyl red and Voges-Proskauer tests were negative. The phylogenetic trees based on the 16S rRNA gene sequences and genome both showed that strain RN3S43 clustered with Ruania alba YIM 93306 and shared 95.5% and < 95.0% of 16S rRNA gene similarities with R. alba YIM 93306 and all the other type strains. MK-8 was the major respiratory quinone. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unidentified lipid were the major polar lipids. The major fatty acids were anteiso-C and iso-C. The genome consisted of a 4,448,708-bp circular chromosome, with a G+C content of 68.2%, predicting 3,911 coding sequence genes, 44 tRNA genes and two rRNA operons. The average nucleotide identity (ANI), amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values of strain RN3S43 to R. alba YIM 93306 were 79.0%, 79.2%, and 23.4%, respectively. The results of phylogenetic, physiological, and phenotypic tests allowed the discrimination of strain RN3S43 from its phylogenetic relatives. Ruania alkalisoli sp. nov. is therefore proposed, and the type strain is RN3S43 (=CGMCC 1.18652 = KCTC 49471).
一株革兰氏阳性、严格需氧、乳白色、杆状细菌菌株，被命名为 RN3S43，从中国内蒙古图们右旗的盐碱土壤中分离得到。RN3S43 菌株在 10-40°C（最佳温度 30°C）、pH6.0-10.0（最佳 pH9.0）和 0-12.5%NaCl（最佳 2-4%）的条件下生长。它氧化酶、过氧化氢酶、脲酶和硝酸盐还原酶均为阳性。甲基红和 Voges-Proskauer 试验均为阴性。基于 16S rRNA 基因序列和基因组构建的系统发育树均表明，菌株 RN3S43 与 Ruania alba YIM 93306 聚为一簇，与 R. alba YIM 93306 和所有其他模式菌株的 16S rRNA 基因相似度均为 95.5%<95.0%。MK-8 为主要呼吸醌。双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未鉴定的脂质为主要极性脂。主要脂肪酸为 anteiso-C 和 iso-C。基因组由一个 4,448,708-bp 的环状染色体组成，G+C 含量为 68.2%，预测有 3,911 个编码序列基因、44 个 tRNA 基因和两个 rRNA 操纵子。RN3S43 与 R. alba YIM 93306 的平均核苷酸同一性（ANI）、氨基酸同一性（AAI）和数字 DNA-DNA 杂交（dDDH）值分别为 79.0%、79.2%和 23.4%。基于系统发育、生理和表型测试的结果，可以将菌株 RN3S43 与它的系统发育近亲区分开来。因此，提出了 Ruania alkalisoli sp. nov.，其模式菌株为 RN3S43（=CGMCC 1.18652=KCTC 49471）。
