Institute of Cash Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, Hebei, China.
Hebei Medicinal Plant Engineering Technology Research Center, Shijiazhuang, Hebei, China.
PLoS One. 2022 Jun 28;17(6):e0269915. doi: 10.1371/journal.pone.0269915. eCollection 2022.
Forsythia suspensa is a traditional Chinese herb. Its numerous metabolites have important roles, as they possessed a wide range of biological activities. This study explored the accumulations of F. suspensa metabolites by performing widely targeted metabolomic analysis. The metabolites were studied at four stages of fruit development. Metabolites in the fruits and leaves of F. suspensa during fruit development included phenolic acids, flavonoids, lipids, lignans and coumarins, amino acids and their derivatives, terpenes, organic acids, nucleotides and their derivatives, alkaloids, quinones, steroids, and tannins. Fourteen Forsythia related metabolites were detected. Their contents varied among the developmental stages. Statistically significant correlations were found between the levels of forsythoside B and 11-methyl-forsythide, and forsythialan B and phillygenin, in both leaves and fruits. According to the correlation analysis between metabolites, Forsythia related metabolites were divided into two classes and five subclasses. In total, 33 compounds presented significant correlations in both fruits and leaves, which indicated the potential relationship in the synthesis of Forsythia related metabolites. Forsythialan B and phillygenin were both negatively correlated with L-valine, while Z-6,7-epoxyligustilid was positively correlated with both compounds. The quality control compounds forsythiaside A and phillyrin were positively and negatively correlated with uracil, respectively. These metabolomics results may facilitate the biosynthesis of Forsythia related metabolites.
连翘是一种传统的中国草药。其众多代谢物具有重要作用，因为它们具有广泛的生物活性。本研究通过进行广泛的靶向代谢组学分析来探索连翘代谢物的积累。在果实发育的四个阶段研究了代谢物。连翘果实和叶片中的代谢物包括酚酸、类黄酮、脂类、木脂素和香豆素、氨基酸及其衍生物、萜类、有机酸、核苷酸及其衍生物、生物碱、醌类、甾体和鞣质。检测到 14 种连翘相关代谢物。它们的含量在发育阶段有所不同。在叶片和果实中均发现连翘苷 B 和 11-甲基连翘苷、连翘苷 B 和连翘脂素之间存在统计学显著相关性。根据代谢物之间的相关性分析，连翘相关代谢物分为两类和五类。共有 33 种化合物在果实和叶片中均呈现显著相关性，这表明连翘相关代谢物的合成存在潜在关系。连翘苷 B 和连翘脂素均与 L-缬氨酸呈负相关，而 Z-6,7-环氧丁香脂素与这两种化合物均呈正相关。质量控制化合物连翘苷 A 和连翘苷与尿嘧啶分别呈正相关和负相关。这些代谢组学结果可能有助于连翘相关代谢物的生物合成。
