Feng Chaohong, Liu Siyu, Jiang Ting, Xu Wei
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.
Molecules. 2023 Nov 17;28(22):7640. doi: 10.3390/molecules28227640.
Although a quadruple mass analyzer and an ion trap mass analyzer have complementary analytical features, they usually have different geometries, operational modes, and electronic control systems. As a continuous effort to extend its coverage, both quadrupole and ion trap operation modes were realized on a "brick" miniature mass spectrometer with a single mass analyzer. In the quadrupole operation mode, low-mass ions ranging from 31 to 502 Th can be analyzed. On the other hand, the ion trap mode can be utilized to cover ions with higher mass to charge ratios (up to 922 Th), as well as performing tandem mass spectrometry. To realize the multiplexing of both operation modes, a printed circuit board (PCB)-based multi-electrode quadrupole-ion trap mass analyzer was designed and integrated in the system. To cover both volatile and non-volatile molecules, two ionization sources were also implemented, including a nano electrospray ionization source and an in-vacuum plasma ionization source. Performances of the instrument operated in these two modes were characterized, such as mass resolution, sensitivity, and mass range. Results demonstrate that the combination of the quadrupole and ion trap operation modes can provide new capabilities when solving analytical problems.
尽管四极杆质量分析器和离子阱质量分析器具有互补的分析特性，但它们通常具有不同的几何结构、操作模式和电子控制系统。为了不断扩大其覆盖范围，在具有单个质量分析器的“砖”式微型质谱仪上实现了四极杆和离子阱两种操作模式。在四极杆操作模式下，可以分析质量范围从31到502 Th的低质量离子。另一方面，离子阱模式可用于覆盖更高质荷比的离子（高达922 Th），并进行串联质谱分析。为了实现两种操作模式的复用，设计了一种基于印刷电路板（PCB）的多电极四极杆 - 离子阱质量分析器并集成到系统中。为了覆盖挥发性和非挥发性分子，还采用了两种电离源，包括纳米电喷雾电离源和真空等离子体电离源。对该仪器在这两种模式下运行的性能进行了表征，如质量分辨率、灵敏度和质量范围。结果表明，四极杆和离子阱操作模式的结合在解决分析问题时可以提供新的能力。
