Neuroimmunoendocrinology Laboratory, Instituto Nacional de Neurología y Neurocirugía "Manuel Velasco Suárez".
Department of Psychiatric Genetics, Clinical Research, Instituto Nacional de Psiquiatría "Ramón de la Fuente Muñiz".
Gac Med Mex. 2022;158(2):104-109. doi: 10.24875/GMM.M22000649.
Fibromyalgia (FM) is a non-degenerative syndrome characterized by generalized, chronic musculoskeletal pain, as well as mood, memory and sleep disorders.
To search for serum anti-neural antibodies (ANeuA) in patients with FM (FMP) in order to rule out autoimmune etiology.
The Fibromyalgia Impact Questionnaire (FIQ) and Beck's depression inventory (BDI) were applied. Immunoreactivity and the target recognized on the sera from FMPs and healthy subjects were analyzed by indirect immunofluorescence and Western blot.
Both FIQ and BDI values were significantly altered in FMPs in comparison with those of controls (FIQ, 70 ± 25 vs. 12 ± 12, p < 0.0001; BDI, 17 ± 11 vs. 4 ± 3, p < 0.0002). Only five out of 15 FMP sera had ANeuA specifically directed against neurons from the medial vestibular nucleus of the brainstem. This immunoreactivity was not detected in the sera from the 14 controls. ANeuA recognized a 45 kDa protein.
30% of FMPs have ANeuA that have not been described before. In future studies, it will be necessary for anti-neural immunoreactivity to be determined in a larger sample and for the role of ANeuAs in the pathophysiology of FM to be established.
纤维肌痛（FM）是一种非退行性综合征，其特征为全身性、慢性肌肉骨骼疼痛以及情绪、记忆和睡眠障碍。
为排除自身免疫病因，我们在纤维肌痛患者（FMP）中寻找血清抗神经抗体（ANeuA）。
应用纤维肌痛影响问卷（FIQ）和贝克抑郁量表（BDI）。通过间接免疫荧光和 Western blot 分析 FMP 和健康受试者血清中的免疫反应性和识别的靶标。
与对照组相比，FMP 的 FIQ 和 BDI 值均显著改变（FIQ，70 ± 25 比 12 ± 12，p < 0.0001；BDI，17 ± 11 比 4 ± 3，p < 0.0002）。在 15 例 FMP 血清中仅有 5 例存在特异性针对脑桥内侧前庭核神经元的 ANeuA。这种免疫反应性在 14 例对照者的血清中未检测到。ANeuA 识别 45 kDa 蛋白。
30%的 FMP 存在以前未描述过的 ANeuA。在未来的研究中，有必要在更大的样本中确定抗神经免疫反应性，并确定 ANeuA 在 FM 病理生理学中的作用。
