Morrissey R L, Zolock D T, Bucci T J, Bikle D D
J Histochem Cytochem. 1978 Aug;26(8):628-34. doi: 10.1177/26.8.357647.
Calcium binding protein (CaBP) was localized by the indirect peroxidase-labeled antibody method in chick duodenum 72 hr after administering 32.5 nmol of cholecalciferol to vitamin D-deficient chicks. CaBP was observed in cytoplasm and nuclei of absorptive cells but was absent from goblet cells. Our results are consistent with the suggested functional role for CaBP in the prevention of intracellular accumulation of calcium by preventing mitochondrial accumulation of calcium, enhancing removal of calcium from absorptive cells, and/or preventing the "leaking" of calcium into cells through the lateral borders. They are not consistent with an extracellular functional role for CaBP.
采用间接过氧化物酶标记抗体法，在给维生素D缺乏的雏鸡投喂32.5纳摩尔胆钙化醇72小时后，对雏鸡十二指肠中的钙结合蛋白（CaBP）进行定位。在吸收细胞的细胞质和细胞核中观察到了CaBP，但杯状细胞中没有。我们的结果与CaBP在预防钙在细胞内积累方面的功能作用相一致，其作用方式包括防止钙在线粒体中积累、增强从吸收细胞中去除钙，和/或防止钙通过细胞侧缘“渗漏”进入细胞。这些结果与CaBP在细胞外的功能作用不一致。
