Jande S S, Tolnai S, Lawson D E
Histochemistry. 1981;71(1):99-116. doi: 10.1007/BF00592574.
Calcium-binding protein (CaBP) has been localized with the immunoperoxidase method using antiserum against purified chick duodenal CaBP. Different preparative procedures were employed to investigate the experimental conditions possibly responsible for the contradictory reports in the literature of the precise cellular localization of CaBP. Freeze substitution, frozen sections followed by fixation and coagulant and non-coagulant fixatives were used with appropriate control sections to demonstrate that the true localization of CaBP in the chick duodenum is in the absorptive cell cytoplasm. The goblet cell localization reported in the literature seems to be a diffusion artifact due to inadequate fixation. CaBP was also localized in several other tissues. In the hen uterus, the tubular glands beneath the surface epithelium showed intense reaction. In the kidney, CaBP was present in the cells of the straight and convoluted segments of distal tubules. The cortex of the chick cerebellum showed the CaBP in Purkinje cells. The entire dendritic trees contained the reaction product. No other neurons in the molecular or the granular layer were stained. In the deep cerebellar nuclei, all neurons were negative and these were outlined by deeply staining axons of the Purkinje cells and their synaptic endings.
利用抗纯化鸡十二指肠钙结合蛋白（CaBP）的抗血清，采用免疫过氧化物酶法对钙结合蛋白进行了定位。采用了不同的制备程序，以研究可能导致文献中关于CaBP精确细胞定位的相互矛盾报道的实验条件。使用冷冻置换、冷冻切片后固定以及凝固剂和非凝固剂固定剂，并设置适当的对照切片，以证明CaBP在鸡十二指肠中的真实定位是在吸收细胞的细胞质中。文献中报道的杯状细胞定位似乎是由于固定不足导致的扩散假象。CaBP也在其他几种组织中被定位。在母鸡子宫中，表面上皮下方的管状腺显示出强烈反应。在肾脏中，CaBP存在于远端小管直段和曲段的细胞中。鸡小脑皮质的浦肯野细胞中显示有CaBP。整个树突都含有反应产物。分子层或颗粒层中的其他神经元均未染色。在小脑深部核团中，所有神经元均为阴性，这些神经元由浦肯野细胞及其突触末梢的深色轴突勾勒出轮廓。
