多重耐药临床分离株外膜通道基因的分子研究
Molecular Investigation of Outer Membrane Channel Genes Among Multidrug Resistance Clinical Isolates.
作者信息
Jasim Al-Thabhawee Maytham Hassan, Muttaleb Al-Dahmoshi Hussein
机构信息
Biology Department, College of Science, University of Babylon, Iraq.
出版信息
Rep Biochem Mol Biol. 2022 Apr;11(1):102-110. doi: 10.52547/rbmb.11.1.102.
BACKGROUND
Multidrug resistance (MDRPA) is most important issue in healthcare setting. It can secrete many virulence effector proteins via its secretion system type (T1SS-T6SS). They are using them as conductor for delivering the effector proteins outside to begins harmful effect on host cell increasing pathogenicity, competition against other microorganism and nutrient acquisition.
METHODS
The study include investigation of 50 isolates of MDRPA for transport secretion system and resistance for antibiotics. Molecular diagnosis using specific primer pairs, investigation of and using specific primer pairs by PCR were also performed.
RESULTS
The results revealed high resistance to beta lactam antibiotics (78% for ceftazidime, 78% for cefepime and 46% for piperacillin) can indicate possessing of isolates for beta lactamases and this confirmed by dropping resistance to piperacillin to 16% when combined with tazobactam. Also, the results shown the ability of MDRPA for pyocyanin biosynthesis using the system of genes.
CONCLUSION
The current study conclude that all isolates of were highly virulent due to their possessing of all transport secretion system to deliver different effector proteins with possible harmful effects of these proteins.
背景
多重耐药铜绿假单胞菌（MDRPA）是医疗环境中最重要的问题。它可通过其1型至6型分泌系统分泌多种毒力效应蛋白。它们利用这些系统将效应蛋白输送到细胞外，从而对宿主细胞产生有害影响，增加致病性、与其他微生物竞争并获取营养。
方法
该研究包括对50株多重耐药铜绿假单胞菌进行转运分泌系统和抗生素耐药性调查。还使用特异性引物对进行分子诊断，通过聚合酶链反应（PCR）对其进行研究。
结果
结果显示对β-内酰胺类抗生素高度耐药（头孢他啶耐药率为78%，头孢吡肟耐药率为78%，哌拉西林耐药率为46%），这可能表明分离株产生了β-内酰胺酶，当与他唑巴坦联合使用时，哌拉西林耐药率降至16%，这证实了上述结论。此外，结果表明多重耐药铜绿假单胞菌能够利用基因系统合成绿脓菌素。
结论
当前研究得出结论，所有分离株均具有高度毒性，因为它们拥有所有转运分泌系统来输送不同的效应蛋白，而这些蛋白可能具有有害作用。
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