Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Engineering Research Center for Color-modulated Extra-sensory Perception Technology, Pusan National University, Busan, South Korea.
J Biophotonics. 2022 Nov;15(11):e202200065. doi: 10.1002/jbio.202200065. Epub 2022 Aug 2.
Histological analysis is widely used to evaluate injured tendons; however, it has the limitation of being semi-quantitative. Hence, we developed a quantification method to objectively evaluate the fibrous structure of tendons, exhibiting the optical property of birefringence, using polarization-sensitive optical coherence tomography (PS-OCT). We used a partial-rupture rat model in which the middle 0.75 cm of the Achilles tendon was cut with a blade. Rats were sacrificed at 2, 4 or 6 weeks after the injury, and PS-OCT and histological analyzes were performed. The PS-OCT phase retardation images and score well represented the structural changes of the injured tendon according to the wound healing state. Therefore, the proposed novel quantification method using PS-OCT can be used to evaluate the fibrous structural status of tendons.
组织学分析被广泛用于评估受伤的肌腱；然而，它具有半定量的局限性。因此，我们开发了一种量化方法，使用偏振敏感光相干断层扫描（PS-OCT）来客观评估具有双折射光学特性的肌腱的纤维结构。我们使用部分断裂大鼠模型，用刀片切断跟腱的中间 0.75 厘米。在损伤后 2、4 或 6 周处死大鼠，并进行 PS-OCT 和组织学分析。PS-OCT 相位延迟图像和评分很好地反映了根据伤口愈合状态受伤肌腱的结构变化。因此，使用 PS-OCT 的新型定量方法可用于评估肌腱的纤维结构状态。
