[α-凝血酶对淋巴细胞DNA合成及排泄的影响]
[Effect of alpha-thrombin on DNA synthesis and excretion by lymphocytes].
作者信息
Barkovskiĭ E V
出版信息
Ukr Biokhim Zh (1978). 1987 Mar-Apr;59(2):33-9.
PMID:3576722
Abstract
Thrombin-activated spleen lymphocytes of mice synthesize 1.5 times more DNA than unstimulated cells. The proportions of palindromic sequences in the newly-synthesized nuclear DNA of control and thrombin-stimulated lymphocytes are 1.1% and 0.5%, respectively. Both the control and thrombin-stimulated lymphocytes release about 10-12% of the newly-synthesized cellular DNA into the culture medium. It is shown that the newly-synthesized DNA of viable, rather than non-viable cells, is the source of extracellular DNA.
摘要
经凝血酶激活的小鼠脾脏淋巴细胞合成的DNA比未受刺激的细胞多1.5倍。对照淋巴细胞和经凝血酶刺激的淋巴细胞新合成的核DNA中回文序列的比例分别为1.1%和0.5%。对照淋巴细胞和经凝血酶刺激的淋巴细胞均将约10 - 12%新合成的细胞DNA释放到培养基中。结果表明，活细胞而非死细胞新合成的DNA是细胞外DNA的来源。
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