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[一种用于薄层层析板上脂质检测的无损灵敏试剂]

[A non-destructive sensitive reagent for lipid detection on TLC plates].

作者信息

Vas'kovskiĭ V E, Berdyshev E V, Dikarev V P

出版信息

Ukr Biokhim Zh (1978). 1987 Mar-Apr;59(2):69-72.

PMID:3576726
Abstract

The aqueous solutions of 3 acidic and 14 basic dyes used as reagents for detection of lipids without their destruction are tested on plates for highly effective TLCh with the silicagel layer fixed by the silicic acid sol. It is shown that 0.002% solution of basic fuchsin in 1% boric acid reveals neutral, phospho- and glycolipids with the sensitivity compared with such in detection of sulphuric acid. It is established that detection of lipids by this reagent with the use of TLCh does not change the composition of their fatty acids.

摘要

将3种酸性染料和14种碱性染料的水溶液用作检测脂质且不破坏脂质的试剂,在涂有由硅酸溶胶固定硅胶层的高效薄层色谱板上进行测试。结果表明,碱性品红在1%硼酸中的0.002%溶液能检测出中性脂质、磷脂和糖脂,其灵敏度与硫酸检测相当。已确定,使用该试剂通过薄层色谱法检测脂质不会改变其脂肪酸组成。

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