Soils and Nutrition Division, Bangladesh Sugarcrop Research Institute, Pabna, 6620, Bangladesh.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South StHaidian District, Beijing, People's Republic of China.
Arch Microbiol. 2022 Jun 29;204(7):437. doi: 10.1007/s00203-022-03064-9.
In this study, a wild-type and five distinct rifampicin-resistant (Rif) rpoB mutants of Pseudomonas stutzeri (i.e., Q518R, D521Y, D521V, H531R and I614T) ability were investigated against harsh environments (particularly nutritional complexity). Among these, the robust Rif phenotype of P. Stutzeri was associated only with base replacements of the amino deposits. The use of carboxylic and amino acids significantly increased in various Rif mutants than that of wild type of P. stutzeri. The assimilation of carbon and nitrogen (N) sources of Rif mutants' confirmed that the organism maintains the adaptation in nutritionally complex environments. Acetylene reduction assay at different times also found the variability for N-fixation in all strains. Among them, the highest nitrogenase activity was determined in mutant 'D521V'. The assimilation of carbon and nitrogen sources of P. stutzeri and its Rif mutants ensures that the organism maintains the adaptability in nutritionally complex environments through fixing more nitrogen.
在这项研究中，研究了五种不同的野生型和利福平抗性（Rif）rpoB 突变体假单胞菌（即 Q518R、D521Y、D521V、H531R 和 I614T）对恶劣环境（特别是营养复杂性）的适应能力。其中，假单胞菌的强大 Rif 表型仅与氨基酸残基的碱基替换有关。与野生型假单胞菌相比，各种 Rif 突变体中羧酸和氨基酸的利用率显著增加。 Rif 突变体对碳氮（N）源的同化证实了该生物在营养复杂的环境中保持适应能力。在不同时间进行的乙炔还原测定也发现所有菌株的固氮能力存在可变性。其中，突变体 'D521V' 中确定的固氮酶活性最高。假单胞菌及其 Rif 突变体对碳氮源的同化确保了该生物通过固定更多的氮来保持在营养复杂环境中的适应性。
