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食欲素-A改变疼痛诱导的环氧化酶-2和脑源性神经营养因子表达的能力与其抑制大鼠辣椒素诱导的牙髓伤害感受的能力相关。

The ability of orexin-A to modify pain-induced cyclooxygenase-2 and brain-derived neurotrophic factor expression is associated with its ability to inhibit capsaicin-induced pulpal nociception in rats.

作者信息

Shahsavari Fatemeh, Abbasnejad Mehdi, Esmaeili-Mahani Saeed, Raoof Maryam

机构信息

Department of Biology, Faculty of Sciences, Shahid Bahonar University, Kerman, Iran.

Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

出版信息

Korean J Pain. 2022 Jul 1;35(3):261-270. doi: 10.3344/kjp.2022.35.3.261.

DOI:10.3344/kjp.2022.35.3.261
PMID:35768981
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9251390/
Abstract

BACKGROUND

The rostral ventromedial medulla (RVM) is a critical region for the management of nociception. The RVM is also involved in learning and memory processes due to its relationship with the hippocampus. The purpose of the present study was to investigate the molecular mechanisms behind orexin-A signaling in the RVM and hippocampus's effects on capsaicin-induced pulpal nociception and cognitive impairments in rats.

METHODS

Capsaicin (100 g) was applied intradentally to male Wistar rats to induce inflammatory pulpal nociception. Orexin-A and an orexin-1 receptor antagonist (SB-334867) were then microinjected into the RVM. Immunoblotting and immunofluorescence staining were used to check the levels of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) in the RVM and hippocampus.

RESULTS

Interdental capsaicin treatment resulted in nociceptive responses as well as a reduction in spatial learning and memory. Additionally, it resulted in decreased BDNF and increased COX-2 expression levels. Orexin-A administration (50 pmol/1 μL/rat) could reverse such molecular changes. SB-334867 microinjection (80 nM/1 μL/rat) suppressed orexin's effects.

CONCLUSIONS

Orexin-A signaling in the RVM and hippocampus modulates capsaicininduced pulpal nociception in male rats by increasing BDNF expression and decreasing COX-2 expression.

摘要

背景

延髓头端腹内侧区（RVM）是伤害感受调控的关键区域。由于其与海马体的关系，RVM也参与学习和记忆过程。本研究的目的是探究RVM中食欲素-A信号传导背后的分子机制以及海马体对辣椒素诱导的大鼠牙髓伤害感受和认知障碍的影响。

方法

将辣椒素（100μg）经牙髓内注射给雄性Wistar大鼠以诱导炎性牙髓伤害感受。然后将食欲素-A和一种食欲素-1受体拮抗剂（SB-334867）微量注射到RVM中。采用免疫印迹和免疫荧光染色法检测RVM和海马体中环氧合酶-2（COX-2）和脑源性神经营养因子（BDNF）的水平。

结果

牙髓内注射辣椒素导致伤害性反应以及空间学习和记忆能力下降。此外，它还导致BDNF水平降低和COX-2表达水平升高。给予食欲素-A（50 pmol/1μL/只大鼠）可逆转这些分子变化。微量注射SB-334867（80 nM/1μL/只大鼠）可抑制食欲素的作用。

结论

RVM和海马体中的食欲素-A信号传导通过增加BDNF表达和降低COX-2表达来调节辣椒素诱导的雄性大鼠牙髓伤害感受。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/f8502d85350d/kjp-35-3-261-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/79fa1297530d/kjp-35-3-261-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/8d10875d50ab/kjp-35-3-261-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/47a1714e1bcd/kjp-35-3-261-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/4a1fe9b3ce90/kjp-35-3-261-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/3ba8c29fe8e9/kjp-35-3-261-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/61cb23348e5f/kjp-35-3-261-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/f8502d85350d/kjp-35-3-261-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/79fa1297530d/kjp-35-3-261-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/cb46d87d2715/kjp-35-3-261-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/8d10875d50ab/kjp-35-3-261-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/47a1714e1bcd/kjp-35-3-261-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/4a1fe9b3ce90/kjp-35-3-261-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/3ba8c29fe8e9/kjp-35-3-261-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/61cb23348e5f/kjp-35-3-261-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9294/9251390/f8502d85350d/kjp-35-3-261-f8.jpg

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