Jackson J A, Andrews J J, Hargis J W
Vet Pathol. 1987 Mar;24(2):129-34. doi: 10.1177/030098588702400205.
Purulent bronchopneumonia was induced in 12 young calves inoculated endotracheally with a total of 2 to 8 X 10(10) viable Haemophilus somnus organisms. Calves treated before inoculation of bacteria with dexamethasone had more extensive lung changes than nontreated calves. Two of six dexamethasone-pretreated calves died while none of the non-pretreated calves died in the 12-day experimental period. Cranial-ventral gray to red lung consolidations with multifocal abscesses were present in all calves. Histologic examination revealed purulent to fibrinopurulent bronchiolitis accompanied by alveolar filling with fibrin, neutrophils, and macrophages. In addition, peribronchiolar fibrosis and bronchiolitis obliterans were common as were interlobular fibrosis and thrombosis of interlobular and pleural lymphatics. Focal to diffuse ulceration of bronchiolar epithelium was present in five of 12 lungs. Abscessation and severe alveolar filling with fibrin, red blood cells, and streaming inflammatory cells were present in dexamethasone-treated calves. H. somnus was isolated from lungs of all 12 calves. Complement fixation and microagglutination antibody titers to H. somnus antigens increased in most calves following infection with H. somnus. This model of H. somnus pneumonia closely resembles the naturally occurring disease.
通过气管内接种总共2至8×10¹⁰个存活的睡眠嗜血杆菌，在12头幼犊中诱发了脓性支气管肺炎。在接种细菌前用 dexamethasone 治疗的犊牛比未治疗的犊牛肺部变化更广泛。在12天的实验期内，6头经dexamethasone预处理的犊牛中有2头死亡，而未预处理的犊牛无一死亡。所有犊牛均出现颅腹侧从灰色到红色的肺实变并伴有多灶性脓肿。组织学检查显示脓性到纤维脓性细支气管炎，伴有肺泡内充满纤维蛋白、中性粒细胞和巨噬细胞。此外，支气管周围纤维化和闭塞性细支气管炎很常见，小叶间纤维化以及小叶间和胸膜淋巴管血栓形成也很常见。12个肺中有5个出现细支气管上皮的局灶性至弥漫性溃疡。在经dexamethasone治疗的犊牛中出现脓肿以及严重的肺泡内充满纤维蛋白、红细胞和流动的炎性细胞。从所有12头犊牛的肺中均分离出睡眠嗜血杆菌。大多数犊牛感染睡眠嗜血杆菌后，针对睡眠嗜血杆菌抗原的补体结合和微凝集抗体滴度升高。这种睡眠嗜血杆菌肺炎模型与自然发生的疾病非常相似。
