Widodo Oky Setyo, Nishihara Saeki, Pambudi Dhidhi, Kusakabe Ken Takeshi, Taura Yasuho, Nishi Yasunobu, Yamato Osamu, Taniguchi Masayasu, Takagi Mitsuhiro
Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi, Japan.
Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Front Vet Sci. 2022 Jun 13;9:828123. doi: 10.3389/fvets.2022.828123. eCollection 2022.
The aim of this study was to verify the association between ovarian size and blood AMH levels in HF cows. Sixty multiparous HF cows from three herds were included in this study. The data required for calculating the ovarian volume included the "major axis (length)," "minor axis (width)," and "thickness" of the ovary. All ultrasonography (US) images were acquired at the outermost ends/poles of both the ovaries and of the follicles (>8 mm) and corpus luteum (CL); concomitantly, the blood was sampled from the jugular or coccygeal vein. Based on the ovarian images of each cow, the following ovarian size patterns were calculated using an image analysis software: (1) total area of both the left and right ovaries, (2) individual size of the large ovary, and (3) individual size of the small ovary. For each ovary area pattern, two properties were assessed: (A) presence of follicles (>8 mm) and CL, which may not secret AMH, in the ovaries and (B) absence of follicles (>8 mm) and CL in the ovaries. Serum AMH levels were measured using enzyme-linked immunosorbent assay. The correlation between ovary size and serum AMH levels was measured in terms of the aforementioned patterns and was evaluated statistically. The results of our preliminary study with ovaries from slaughter-house cows ( = 22) revealed that the "thickness" of the ovary was not necessary for estimating ovarian volume and that length and width were sufficient. A strong correlation was observed among ovarian length, width, and thickness (r > 0.96). No significant difference was observed ( > 0.05) in the mean ages or parities among the three herds. Among the ovary sizes measured in this study, the highest correlation was found between the total size of an individual large ovary (including follicular and luteal size) and AMH levels (r = 0.387, = 0.002). This is the first study to demonstrate the correlation between total size of individual large ovaries and serum AMH levels in HF cows. US observations of the ovaries will allow for estimation of differences in AMH levels and help predict ovarian activity and superovulation performance of cows.
本研究的目的是验证荷斯坦弗里生（HF）奶牛卵巢大小与血液抗缪勒氏管激素（AMH）水平之间的关联。本研究纳入了来自三个牛群的60头经产HF奶牛。计算卵巢体积所需的数据包括卵巢的“长轴（长度）”“短轴（宽度）”和“厚度”。所有超声（US）图像均在两侧卵巢以及卵泡（>8mm）和黄体（CL）的最外端/极点处采集；与此同时，从颈静脉或尾静脉采集血液。根据每头奶牛的卵巢图像，使用图像分析软件计算以下卵巢大小模式：（1）左右卵巢的总面积，（2）大卵巢的个体大小，（3）小卵巢的个体大小。对于每种卵巢面积模式，评估两个属性：（A）卵巢中存在可能不分泌AMH的卵泡（>8mm）和CL，以及（B）卵巢中不存在卵泡（>8mm）和CL。使用酶联免疫吸附测定法测量血清AMH水平。根据上述模式测量卵巢大小与血清AMH水平之间的相关性，并进行统计学评估。我们对屠宰场奶牛卵巢（n = 22）的初步研究结果表明，卵巢的“厚度”对于估计卵巢体积并非必需，长度和宽度就足够了。卵巢长度、宽度和厚度之间观察到强相关性（r>0.96）。三个牛群之间的平均年龄或胎次未观察到显著差异（P>0.05）。在本研究测量的卵巢大小中，发现个体大卵巢的总大小（包括卵泡和黄体大小）与AMH水平之间的相关性最高（r = 0.387，P = 0.002）。这是第一项证明HF奶牛个体大卵巢总大小与血清AMH水平之间相关性的研究。对卵巢的超声观察将有助于估计AMH水平的差异，并有助于预测奶牛的卵巢活动和超排卵性能。
