Gamarra G, Ponsart C, Lacaze S, Le Guienne B, Humblot P, Deloche M-C, Monniaux D, Ponter A A
UNCEIA Département Recherche et Développement, 13 rue Jouet, F-94704 Maisons-Alfort, France.
MIDATEST, Domine de Sensacq cedex 55A, F-64230 Denguin, France.
Reprod Fertil Dev. 2015 Nov;27(8):1249-61. doi: 10.1071/RD14091.
Rapid genetic improvement in cattle requires the production of high numbers of embryos of excellent quality. Increasing circulating insulin and/or glucose concentrations improves ovarian follicular growth, which may improve the response to superovulation. The measurement of anti-Müllerian hormone (AMH) can help predict an animal's response to superovulation treatment. The aim of the present study was to investigate whether increasing circulating insulin concentrations, through propylene glycol (PG) drenches, could improve in vitro embryo production in oestrus-synchronised superovulated heifers with different AMH profiles. Holstein heifers were grouped according to pre-experimental AMH concentrations as low (L) or high (H). The PG drench increased circulating insulin and glucose concentrations and reduced β-hydroxybutyrate and urea concentrations compared with the control group. AMH was a good predictor of follicle and oocyte numbers at ovum pick-up (OPU), and of oocyte and embryo quality (AMH H>AMH L). PG in the AMH H group increased the number of follicles and blastocyst quality above that in the control group, but did not improve these parameters in the AMH L group. These results indicate that short-term oral PG supplementation modifies an animal's metabolic milieu and is effective in improving in vitro embryo production, after superovulation-OPU, more markedly in heifers with high rather than low AMH concentrations.
牛的快速遗传改良需要大量生产优质胚胎。提高循环胰岛素和/或葡萄糖浓度可改善卵巢卵泡生长，这可能会提高对超数排卵的反应。抗苗勒管激素（AMH）的测量有助于预测动物对超数排卵治疗的反应。本研究的目的是调查通过丙二醇（PG）灌服增加循环胰岛素浓度是否能改善不同AMH水平的发情同步超排小母牛的体外胚胎生产。荷斯坦小母牛根据实验前的AMH浓度分为低（L）或高（H）组。与对照组相比，PG灌服增加了循环胰岛素和葡萄糖浓度，降低了β-羟丁酸和尿素浓度。AMH是采卵（OPU）时卵泡和卵母细胞数量以及卵母细胞和胚胎质量的良好预测指标（AMH H>AMH L）。AMH H组的PG使卵泡数量和囊胚质量高于对照组，但在AMH L组中未改善这些参数。这些结果表明，短期口服PG补充剂可改变动物的代谢环境，在超排-OPU后有效提高体外胚胎生产，在AMH浓度高的小母牛中比低浓度的更明显。
