Qin Yuxi, Zhao Baojin, Deng Huifang, Zhang Mengjiao, Qiao Yanan, Liu Qiling, Shi Chuandao, Li Yunlan
School of Public Health, Shaanxi University of Chinese medicine, Xi'an, China.
School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China.
Front Pharmacol. 2022 Jun 13;13:890148. doi: 10.3389/fphar.2022.890148. eCollection 2022.
Flavonoids were the major phytochemicals against hepatic peroxidative injury in (Willd.) R. J. Wang with an inventive bio-enzymatic method by our group (LU500041). Firstly, the total flavonoids from (Willd.) R. J. Wang were extracted by reflux, ultrasonic, ultrasound-assisted enzymatic methods (TFH), and the bio-enzymatic method (Ey-TFH). Then 24 flavonoid compounds were isolated and quantified in the extracts by UPLC-MS/MS. Next, six representative differential compounds in Ey-TFH were further screened out by multivariate statistical analysis compared with those in TFH. In a further step, Ey-TFH presented a higher protective rate (59.30 ± 0.81%) against HO-damaged HL-02 hepatocytes than TFH. And six representative differential compounds at 8 and 16 μmol/L all exerted significant hepatoprotective effects ( < 0.05 or < 0.01). Finally, the therapeutic action of Ey-TFH for nonalcoholic fatty liver disease (NAFLD) was processed by a rat's model induced with a high-fat diet. Ey-TFH (90, 120 mg/kg) significantly ameliorated the lipid accumulation in the rat model ( < 0.05). Meanwhile, Ey-TFH relieved liver damage. The levels of ALT, ALP, AST, LDH, and γ-GT in rats' serum were also significantly reduced ( 0.05 or 0.01). In addition to this, the body's antioxidant capacity was improved with elevated SOD and GSH levels ( 0.05) and down-regulated MDA content ( 0.01) after Ey-TFH administration. Histopathological observations of staining confirmed the hepatic-protective effect of Ey-TFH.
黄酮类化合物是我们团队采用创新生物酶法（LU500041）对抗（Willd.）R. J. Wang肝过氧化损伤的主要植物化学物质。首先，通过回流、超声、超声辅助酶法（TFH）和生物酶法（Ey-TFH）从（Willd.）R. J. Wang中提取总黄酮。然后通过UPLC-MS/MS对提取物中的24种黄酮类化合物进行分离和定量。接下来，通过多变量统计分析，与TFH中的化合物相比，进一步筛选出Ey-TFH中的六种代表性差异化合物。进一步的研究表明，Ey-TFH对HO损伤的HL-02肝细胞的保护率（59.30±0.81%）高于TFH。8和16μmol/L的六种代表性差异化合物均具有显著的肝保护作用（P<0.05或P<0.01）。最后，通过高脂饮食诱导的大鼠模型研究Ey-TFH对非酒精性脂肪性肝病（NAFLD）的治疗作用。Ey-TFH（90、120mg/kg）显著改善了大鼠模型中的脂质蓄积（P<0.05）。同时，Ey-TFH减轻了肝脏损伤。大鼠血清中ALT、ALP、AST、LDH和γ-GT水平也显著降低（P<0.05或P<0.01）。此外，Ey-TFH给药后，随着SOD和GSH水平升高（P<0.05）和MDA含量下调（P<0.01），机体的抗氧化能力得到改善。染色的组织病理学观察证实了Ey-TFH的肝脏保护作用。
