Skala Melissa, Ramanujam Nirmala
Department of Biomedical Engineering, Duke University, CIEMAS, Durham, NC, USA.
Methods Mol Biol. 2010;594:155-62. doi: 10.1007/978-1-60761-411-1_11.
Metabolic monitoring at the cellular level in live tissues is important for understanding cell function, disease processes, and potential therapies. Multiphoton imaging of the relative amounts of NADH and FAD (the primary electron donor and acceptor, respectively, in the electron transport chain) provides a noninvasive method for monitoring cellular metabolic activity with high resolution in three dimensions in vivo. NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo.
在活组织中进行细胞水平的代谢监测对于理解细胞功能、疾病进程和潜在治疗方法至关重要。对NADH和FAD（分别是电子传递链中的主要电子供体和受体）的相对含量进行多光子成像，提供了一种在体内以三维高分辨率监测细胞代谢活性的非侵入性方法。NADH和FAD是内源性组织荧光团，因此该方法不需要外源性染色或组织切除。我们描述了体内多光子氧化还原比率成像的原理和方案。
