Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
Department of Obstetrics and Gynecology, University of Kinshasa, Kinshasa, D. R. Congo.
Am J Reprod Immunol. 2023 Jun;89(6):e13591. doi: 10.1111/aji.13591. Epub 2022 Aug 15.
This study aimed to identify subsets of regulatory T cells (Tregs) associated with ovarian aging and determine whether they can be used as markers of reproductive aging.
This prospective cohort study was conducted among women of reproductive age. Basic physiological characteristics, reproductive hormones, Treg cell subsets, and correlations between these parameters were assessed. The POSEIDON criteria was used to identify women with low reproductive potential.
The percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells significantly increased with age. Women between 40 and 49 years had significantly higher percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells than those at 20-29, 30-34, and 35-39 years old. Age positively correlated with FSH levels and the percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells, but inversely correlated with antral follicle count (AFC) and AMH levels. Interestingly, a positive correlation was found between the percentages of HLA-DR CD45RA- Tregs and FSH levels, whereas an inverse correlation was found between those of HLA-DR CD45RA- Tregs and AFC or AMH levels. Furthermore, a significant positive correlation was observed between the percentages of CD28- Treg-like cells and AFC. Based on POSEIDON criteria, women with the percentages of HLA-DR CD45RA- Tregs and CD28- Treg-like cells above reference value ranges were assigned to the low prognosis groups.
These findings suggest that HLA-DR CD45RA- Tregs and CD28- Treg-like cells can be used as immunologic markers of reproductive aging, which helps clinicians identify women with low reproductive potential and establish individualized therapeutic strategies.
本研究旨在确定与卵巢衰老相关的调节性 T 细胞（Treg）亚群，并确定它们是否可用作生殖衰老的标志物。
这项前瞻性队列研究在育龄妇女中进行。评估了基本生理特征、生殖激素、Treg 细胞亚群以及这些参数之间的相关性。采用 POSEIDON 标准来识别生育能力低下的女性。
HLA-DR CD45RA-Tregs 和 CD28-Treg 样细胞的百分比随年龄增长而显著增加。40-49 岁的女性与 20-29、30-34 和 35-39 岁的女性相比，HLA-DR CD45RA-Tregs 和 CD28-Treg 样细胞的百分比显著更高。年龄与 FSH 水平以及 HLA-DR CD45RA-Tregs 和 CD28-Treg 样细胞的百分比呈正相关，与窦卵泡计数（AFC）和 AMH 水平呈负相关。有趣的是，HLA-DR CD45RA-Tregs 的百分比与 FSH 水平呈正相关，而与 AFC 或 AMH 水平呈负相关。此外，CD28-Treg 样细胞的百分比与 AFC 之间存在显著的正相关。根据 POSEIDON 标准，将 HLA-DR CD45RA-Tregs 和 CD28-Treg 样细胞百分比高于参考值范围的女性分配到低预后组。
这些发现表明，HLA-DR CD45RA-Tregs 和 CD28-Treg 样细胞可作为生殖衰老的免疫标志物，有助于临床医生识别生育能力低下的女性，并制定个体化的治疗策略。
