Department of Mechanical Engineering, Anna University, Chennai, Tamil Nadu, India.
Biomed Phys Eng Express. 2022 Jul 11;8(5). doi: 10.1088/2057-1976/ac7d76.
Electro-spinnable polymeric materials can easily form two-dimensional (2D) nanofibrous scaffolds improving biochemical functionalities specially in the area of skin wound healing and nanomedicine, but it has been hard to achieve this on a highly mechanically stable biopolymer, Poly ether ether ketone (PEEK), due to its intrinsic hydrophobicity and chemical inertness. Herein, we demonstrated a novel nanomedicine healing system consisting of sulphonated poly ether ether ketone combined with(SPEEK + RSV), which could act as an effective 2D nano bio-materialand, without observable cytotoxicity. The fabricated nanocomposites exhibited enriched skin cell proliferation and adhesion as confirmed from the results of MTT, cell adhesion and live-dead assay. Results of SEM analysis showed a uniform nano-sized distribution with adequate pore size and porosity % facilitating a desired breathable environment at the wound site. The results of FT-IR, tensile studies and TGA analyses confirmed the presence of appropriate bonds and improved mechanical stability of theincorporated nanofibrous scaffold. Results of anti-microbial analysis portrayed good potentiality of the fabricated nanofibers in treating wounds colonized with bacterial infections. Controlled drug release of resveratrol established the bio-compatibility of the nanofibers in skin wound regeneration.analysis assessed in female Wistar rats enabled complete wound closure with 100% wound contraction within 16 days. Results of histopathology analysis through H-E and MT staining presented the re-surfing of the wound environment with regeneration of epithelium, granulation tissue and collagen. Thus, the fabricated 2D nanofibrous scaffold incorporated with pharmaceutical RSV bio-medicine perceptively mimicked skin ECM convincingly aiding the progression of skin wound regeneration mechanism.
可电纺的聚合材料很容易形成二维（2D）纳米纤维支架，可改善生化功能，尤其在皮肤创伤愈合和纳米医学领域，但由于其内在的疏水性和化学惰性，很难在高度机械稳定的生物聚合物聚醚醚酮（PEEK）上实现这一目标。在这里，我们展示了一种由磺化聚醚醚酮与 RSV 结合而成的新型纳米医学治疗系统（SPEEK+RSV），它可以作为一种有效的 2D 纳米生物材料，且没有明显的细胞毒性。从 MTT、细胞黏附和死活检测的结果可以证实，所制备的纳米复合材料表现出丰富的皮肤细胞增殖和黏附能力。SEM 分析结果显示，纳米复合材料具有均匀的纳米级分布、充足的孔径和孔隙率%，为伤口部位提供了理想的透气环境。FT-IR、拉伸研究和 TGA 分析的结果证实了适当的键的存在和增强了所掺入的纳米纤维支架的机械稳定性。抗菌分析的结果表明，所制备的纳米纤维在治疗受细菌感染的伤口方面具有良好的潜力。白藜芦醇的控制药物释放证实了纳米纤维在皮肤创伤再生中的生物相容性。在雌性 Wistar 大鼠中的受控药物释放研究评估表明，在 16 天内，完全闭合伤口，实现 100%的伤口收缩。通过 H-E 和 MT 染色的组织病理学分析结果表明，伤口环境得到了重新修复，上皮、肉芽组织和胶原蛋白得到了再生。因此，所制备的二维纳米纤维支架与药物 RSV 生物医学相结合，令人信服地模拟了皮肤细胞外基质，有助于皮肤创伤再生机制的进展。
