Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.
Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China.
J Glob Antimicrob Resist. 2022 Sep;30:228-230. doi: 10.1016/j.jgar.2022.06.023. Epub 2022 Jun 28.
The occurrence of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) has been increasing in China during the last five years. The bla-carrying CRKP strain's clonal propagation can readily lead to nosocomial epidemics. Here, we report the genome sequence of an OXA-232 and CTX-M-15 co-producing K. pneumoniae strain isolated from a gallbladder infection in China.
The genome sequence of K. pneumoniae S105 was determined using the Illumina NovaSeq 6000 platform. Antimicrobial resistance genes (ARGs), multilocus sequence typing (MLST) and plasmid replicons were identified using the BacWGSTdb server. The phylogenetic relationship between S105 and other K. pneumoniae strains was analysed using the core genome multilocus sequence typing (cgMLST) strategy.
The genomic sequence of K. pneumoniae S105 is made up of 111 contigs with a total length of 5 748 752 bp. According to the Pasteur MLST scheme, S105 belongs to sequence type (ST) 15. Fifteen ARGs were discovered in the genome, including the beta-lactam resistance genes bla and bla. The bla gene was located in a ColKP3 plasmid. KL112 was anticipated to be the capsule and lipopolysaccharide serotype. A total of 73 phylogenetically related strains were found from 19 nations across four continents; 22 of them were from China, with 21 strains harbouring the bla gene, and the majority of them diverged by just 6-37 cgMLST alleles.
In summary, we reported the genomic sequencing of a K. pneumoniae ST15 clinical strain co-carrying the bla and bla genes. The clonal dissemination of OXA-232-producing K. pneumoniae ST15 strains in China needs our attention.
过去五年，中国产 OXA-232 的碳青霉烯类耐药肺炎克雷伯菌（CRKP）的发生率一直在增加。携带 bla 的 CRKP 菌株的克隆传播很容易导致医院内的流行。在此，我们报告了一株产 OXA-232 和 CTX-M-15 的肺炎克雷伯菌 S105 从中国胆囊感染中分离出来的基因组序列。
使用 Illumina NovaSeq 6000 平台对肺炎克雷伯菌 S105 的基因组进行测序。使用 BacWGSTdb 服务器鉴定抗生素耐药基因（ARGs）、多位点序列分型（MLST）和质粒复制子。使用核心基因组多位点序列分型（cgMLST）策略分析 S105 与其他肺炎克雷伯菌菌株的系统发育关系。
肺炎克雷伯菌 S105 的基因组序列由 111 个长度为 5748752bp 的片段组成。根据巴斯德 MLST 方案，S105 属于序列型（ST）15。在基因组中发现了 15 个 ARGs，包括 bla 和 bla 等β-内酰胺类耐药基因。bla 基因位于 ColKP3 质粒上。KL112 被预测为荚膜和脂多糖血清型。从四大洲 19 个国家共发现了 73 株具有系统发育关系的菌株；其中 22 株来自中国，携带 bla 基因的菌株有 21 株，其中大多数菌株的 cgMLST 等位基因仅相差 6-37 个。
综上所述，我们报告了一株同时携带 bla 和 bla 基因的肺炎克雷伯菌 ST15 临床株的基因组测序。产 OXA-232 的肺炎克雷伯菌 ST15 在中国的克隆传播值得关注。
