Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China.
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.
J Glob Antimicrob Resist. 2020 Mar;20:339-341. doi: 10.1016/j.jgar.2020.01.021. Epub 2020 Mar 2.
The resistance rate of Klebsiella pneumoniae to commonly used antibiotics has been increasing rapidly, causing serious concern among clinicians and microbiologists. Resistance to carbapenems in K. pneumoniae is increasing dramatically in Chinese hospitals. Here we report the genome sequence of an NDM-1 and KPC-2 co-producing K. pneumoniae (CRKP380) isolated from a bloodstream infection in Hangzhou, China.
The whole genome sequence of strain CRKP380 was determined using an Illumina NovaSeq 6000 platform. Antimicrobial resistance genes (ARGs) were identified using the BacWGSTdb server. The phylogenetic relationship between strain CRKP380 and other K. pneumoniae strains isolated from Hangzhou currently deposited in the NCBI GenBank database was analysed using a core genome-based single nucleotide polymorphism strategy.
Klebsiella pneumoniae CRKP380 was resistant to all antibiotics tested except tigecycline and colistin. The genome sequence of K. pneumoniae CRKP380 consisted of 75 contigs comprising 5 590 460 bp. According to the Pasteur multilocus sequence typing (MLST) scheme, CRKP380 belongs to ST11. Eight ARGs were identified in CRKP380, including two carbapenemase genes (bla and bla). Ten phylogenetically-related K. pneumoniae strains from Hangzhou were identified with identical ARGs and the same capsular serotype KL105, but none of these strains carried the bla gene.
Here we report the genome sequence of a K. pneumoniae ST11 clinical strain co-carrying bla and bla from Hangzhou, China. The genome sequence of CRKP380 can be used as a reference sequence for future comparative genomic analysis, including the acquisition and mobilisation of carbapenem resistance genes.
肺炎克雷伯菌对常用抗生素的耐药率迅速上升，引起临床医生和微生物学家的严重关注。中国医院中产碳青霉烯酶肺炎克雷伯菌（CRKP）对碳青霉烯类的耐药性呈显著上升趋势。本研究报告了一株来源于中国杭州血流感染的产 NDM-1 和 KPC-2 的肺炎克雷伯菌（CRKP380）的基因组序列。
采用 Illumina NovaSeq 6000 平台测定 CRKP380 全基因组序列，利用 BacWGSTdb 服务器鉴定耐药基因，采用基于核心基因组单核苷酸多态性的策略分析 CRKP380 与目前已在 NCBI GenBank 数据库中存储的来自杭州的其他肺炎克雷伯菌菌株的系统发育关系。
肺炎克雷伯菌 CRKP380 对除替加环素和黏菌素外的所有测试抗生素均耐药。CRKP380 的基因组序列由 75 个序列组成，共包含 5590460bp。根据巴斯德多位点序列分型（MLST）方案，CRKP380 属于 ST11 型。CRKP380 共鉴定出 8 个耐药基因，包括两个碳青霉烯酶基因（bla 和 bla）。从杭州鉴定出 10 株与 CRKP380 具有相同 ARGs 和相同荚膜血清型 KL105 的亲缘关系密切的肺炎克雷伯菌菌株，但这些菌株均不携带 bla 基因。
本研究报告了一株来自中国杭州的同时携带 bla 和 bla 的肺炎克雷伯菌 ST11 临床株的基因组序列。CRKP380 的基因组序列可作为未来比较基因组分析的参考序列，包括碳青霉烯类耐药基因的获取和转移。
