Basic Medical Science Department, School of Medicine, The Hashemite University, Zarqa, Jordan.
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, NE7 7DN, UK.
Sci Rep. 2022 Jun 30;12(1):11114. doi: 10.1038/s41598-022-15375-4.
Studies of microbiota reveal inter-relationships between the microbiomes of the gut and lungs. This relationship may influence the progression of lung disease, particularly in patients with cystic fibrosis (CF), who often experience extraoesophageal reflux (EOR). Despite identifying this relationship, it is not well characterised. Our hypothesis is that the gastric and lung microbiomes in CF are related, with the potential for aerodigestive pathophysiology. We evaluated gastric and sputum bacterial communities by culture and 16S rRNA gene sequencing in 13 CF patients. Impacts of varying levels of bile acids, pepsin and pH on patient isolates of Pseudomonas aeruginosa (Pa) were evaluated. Clonally related strains of Pa and NTM were identified in gastric and sputum samples from patients with symptoms of EOR. Bacterial diversity was more pronounced in sputa compared to gastric juice. Gastric and lung bile and pepsin levels were associated with Pa biofilm formation. Analysis of the aerodigestive microbiomes of CF patients with negative sputa indicates that the gut can be a reservoir of Pa and NTM. This combined with the CF patient's symptoms of reflux and potential aspiration, highlights the possibility of communication between microorganisms of the gut and the lungs. This phenomenon merits further research.
研究微生物组揭示了肠道和肺部微生物组之间的相互关系。这种关系可能会影响肺部疾病的进展，特别是在经常经历食管外反流（EOR）的囊性纤维化（CF）患者中。尽管已经确定了这种关系，但它的特征尚未得到很好的描述。我们的假设是 CF 患者的胃和肺部微生物组是相关的，具有潜在的气液生理病理学。我们通过培养和 16S rRNA 基因测序评估了 13 名 CF 患者的胃和痰液细菌群落。评估了不同水平的胆汁酸、胃蛋白酶和 pH 值对铜绿假单胞菌（Pa）患者分离株的影响。在有 EOR 症状的患者的胃和痰液样本中，鉴定出了与 Pa 和非结核分枝杆菌（NTM）相关的克隆相关菌株。与胃液相比，痰液中的细菌多样性更为明显。胃和肺部的胆汁和胃蛋白酶水平与 Pa 生物膜形成有关。对痰液阴性的 CF 患者的气液微生物组进行分析表明，肠道可以成为 Pa 和 NTM 的储库。再加上 CF 患者的反流和潜在的吸入症状，突显了肠道和肺部微生物之间进行交流的可能性。这种现象值得进一步研究。
