Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA.
Department of Infectious Diseases Research, Korea Research Institute of Chemical Technology, Daejeon, South Korea.
Methods Mol Biol. 2022;2507:375-387. doi: 10.1007/978-1-0716-2368-8_20.
The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.
苯乙烯马来酸（SMA）和异丁烯马来酸（DIBMA）共聚物的开发为传统去污剂提取整体膜蛋白提供了一种替代方法。这些聚合物通过插入膜中，可以以多（苯乙烯共马来酸酐）衍生物形成的天然纳米盘的形式提取膜蛋白和脂质。与去污剂溶解不同，在去污剂溶解中，膜蛋白可能会失去对正确折叠和稳定性至关重要的环形脂质，而天然纳米盘允许蛋白质存在于天然脂质环境中。此外，基于聚合物的纳米盘可以使用类似于用去污剂溶解纯化建立的方案的常见色谱方法进行纯化。在这里，我们描述了使用几种商业共聚物对整体膜蛋白进行溶解筛选和纯化。
