清洁剂替代品：使用合成纳米盘聚合物进行膜蛋白纯化。
Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.
机构信息
Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA.
Department of Infectious Diseases Research, Korea Research Institute of Chemical Technology, Daejeon, South Korea.
出版信息
Methods Mol Biol. 2022;2507:375-387. doi: 10.1007/978-1-0716-2368-8_20.
Abstract
The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.
摘要
苯乙烯马来酸（SMA）和异丁烯马来酸（DIBMA）共聚物的开发为传统去污剂提取整体膜蛋白提供了一种替代方法。这些聚合物通过插入膜中，可以以多（苯乙烯共马来酸酐）衍生物形成的天然纳米盘的形式提取膜蛋白和脂质。与去污剂溶解不同，在去污剂溶解中，膜蛋白可能会失去对正确折叠和稳定性至关重要的环形脂质，而天然纳米盘允许蛋白质存在于天然脂质环境中。此外，基于聚合物的纳米盘可以使用类似于用去污剂溶解纯化建立的方案的常见色谱方法进行纯化。在这里，我们描述了使用几种商业共聚物对整体膜蛋白进行溶解筛选和纯化。
相似文献
1
Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.清洁剂替代品：使用合成纳米盘聚合物进行膜蛋白纯化。
Methods Mol Biol. 2022;2507:375-387. doi: 10.1007/978-1-0716-2368-8_20.
2
Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer.使用二异丁烯/马来酸共聚物将膜蛋白增溶到功能性脂质双层纳米盘中。
Angew Chem Int Ed Engl. 2017 Feb 6;56(7):1919-1924. doi: 10.1002/anie.201610778. Epub 2017 Jan 12.
3
The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes.苯乙烯-马来酸共聚物对可及和不可及膜中整合膜蛋白的增溶作用。
Biochim Biophys Acta Biomembr. 2017 Oct;1859(10):2133-2143. doi: 10.1016/j.bbamem.2017.07.011. Epub 2017 Jul 24.
4
Influence of DIBMA Polymer Length on Lipid Nanodisc Formation and Membrane Protein Extraction.DIBMA 聚合物长度对脂质纳米盘形成和膜蛋白提取的影响。
Biomacromolecules. 2021 Feb 8;22(2):763-772. doi: 10.1021/acs.biomac.0c01538. Epub 2020 Dec 29.
5
Alternatives to Styrene- and Diisobutylene-Based Copolymers for Membrane Protein Solubilization via Nanodisc Formation.通过纳米盘形成用于膜蛋白增溶的苯乙烯和异丁烯基共聚物的替代品。
Angew Chem Int Ed Engl. 2023 Oct 23;62(43):e202306572. doi: 10.1002/anie.202306572. Epub 2023 Sep 19.
6
A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification.SMA（苯乙烯马来酸）和 DIBMA（二异丁烯马来酸）在膜蛋白纯化方面的比较。
Biochim Biophys Acta Biomembr. 2020 Jul 1;1862(7):183281. doi: 10.1016/j.bbamem.2020.183281. Epub 2020 Mar 21.
7
Detergent-Free Membrane Protein Purification Using SMA Polymer.无去污剂的 SMA 聚合物膜蛋白纯化。
Methods Mol Biol. 2022;2507:389-404. doi: 10.1007/978-1-0716-2368-8_21.
8
Structure of Diisobutylene Maleic Acid Copolymer (DIBMA) and Its Lipid Particle as a "Stealth" Membrane-Mimetic for Membrane Protein Research.二异丁烯马来酸共聚物（DIBMA）及其脂质颗粒的结构作为用于膜蛋白研究的“隐形”膜模拟物
ACS Appl Bio Mater. 2021 Jun 21;4(6):4760-4768. doi: 10.1021/acsabm.0c01626. Epub 2021 Apr 6.
9
Lipid packing is disrupted in copolymeric nanodiscs compared with intact membranes.与完整的细胞膜相比，脂双层在共聚物纳米盘中被打乱。
Biophys J. 2023 Jun 6;122(11):2256-2266. doi: 10.1016/j.bpj.2023.01.013. Epub 2023 Jan 14.
10
Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene-maleic acid copolymers.影响苯乙烯-马来酸共聚物从大肠杆菌膜中溶解膜蛋白的因素。
Biochim Biophys Acta Biomembr. 2020 Feb 1;1862(2):183125. doi: 10.1016/j.bbamem.2019.183125. Epub 2019 Nov 15.
引用本文的文献
1
Advances in solubilization and stabilization techniques for structural and functional studies of membrane proteins.用于膜蛋白结构与功能研究的增溶和稳定化技术进展
PeerJ. 2025 Apr 4;13:e19211. doi: 10.7717/peerj.19211. eCollection 2025.
2
The Evolution of ABC Importers.ABC进口商的发展历程
J Mol Biol. 2025 Jun 1;437(11):169082. doi: 10.1016/j.jmb.2025.169082. Epub 2025 Mar 13.
3
GABA receptor π forms channels that stimulate ERK through a G-protein-dependent pathway.γ-氨基丁酸（GABA）受体π形成的通道通过G蛋白依赖性途径刺激细胞外信号调节激酶（ERK）。
Mol Cell. 2025 Jan 2;85(1):166-176.e5. doi: 10.1016/j.molcel.2024.11.016. Epub 2024 Dec 5.
4
Structural mass spectrometry approaches to understand multidrug efflux systems.结构质谱方法研究多药外排系统。
Essays Biochem. 2023 Mar 29;67(2):255-267. doi: 10.1042/EBC20220190.
5
The Myth of The Annular Lipids.环状脂质的神话
Biomedicines. 2022 Oct 22;10(11):2672. doi: 10.3390/biomedicines10112672.
6
Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment.无去污剂分离膜蛋白及其在近天然膜环境中研究它们的策略。
Biomolecules. 2022 Aug 4;12(8):1076. doi: 10.3390/biom12081076.
本文引用的文献
1
Cryo-EM structure of the octameric pore of Clostridium perfringens β-toxin.产气荚膜梭菌β 毒素八聚体孔的冷冻电镜结构。
EMBO Rep. 2022 Dec 6;23(12):e54856. doi: 10.15252/embr.202254856. Epub 2022 Oct 10.
2
Conformational trapping of an ABC transporter in polymer lipid nanoparticles.聚合物脂质纳米粒中 ABC 转运蛋白的构象捕获。
Biochem J. 2022 Jan 28;479(2):145-159. doi: 10.1042/BCJ20210312.
3
A bioinspired glycopolymer for capturing membrane proteins in native-like lipid-bilayer nanodiscs.一种仿生糖聚合物，用于在类似天然的脂质双层纳米盘中捕获膜蛋白。
Nanoscale. 2022 Feb 3;14(5):1855-1867. doi: 10.1039/d1nr03811g.
4
Cryo-EM Structure of Mechanosensitive Channel YnaI Using SMA2000: Challenges and Opportunities.使用SMA2000解析机械敏感通道YnaI的冷冻电镜结构：挑战与机遇
Membranes (Basel). 2021 Oct 31;11(11):849. doi: 10.3390/membranes11110849.
5
Cryo-EM structures of cytochrome reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site.细胞色素 c 的冷冻电镜结构显示在一个动态的底物结合位点中存在结合的磷脂和泛醌-8。
Proc Natl Acad Sci U S A. 2021 Aug 24;118(34). doi: 10.1073/pnas.2106750118.
6
Structure and function at the lipid-protein interface of a pentameric ligand-gated ion channel.五聚体配体门控离子通道的脂-蛋白界面的结构与功能。
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). doi: 10.1073/pnas.2100164118.
7
CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution.CryoEM 结构的抗菌靶标 PBP1b 在 3.3 Å 分辨率。
Nat Commun. 2021 May 13;12(1):2775. doi: 10.1038/s41467-021-23063-6.
8
Structural biology of endogenous membrane protein assemblies in native nanodiscs.天然纳米盘内源性膜蛋白组装体的结构生物学。
Curr Opin Struct Biol. 2021 Aug;69:70-77. doi: 10.1016/j.sbi.2021.03.008. Epub 2021 Apr 27.
9
Detergent-free purification and reconstitution of functional human serotonin transporter (SERT) using diisobutylene maleic acid (DIBMA) copolymer.无洗涤剂法纯化和重构功能人血清素转运体（SERT）使用二异丁烯马来酸（DIBMA）共聚物。
Biochim Biophys Acta Biomembr. 2021 Jul 1;1863(7):183602. doi: 10.1016/j.bbamem.2021.183602. Epub 2021 Mar 18.
10
Mechanism of gating and partial agonist action in the glycine receptor.甘氨酸受体门控和部分激动剂作用机制。
Cell. 2021 Feb 18;184(4):957-968.e21. doi: 10.1016/j.cell.2021.01.026. Epub 2021 Feb 9.
Zotero 插件