Department of Ophthalmology, Xinjiang Hotan District People's Hospital, Ho tian, PR China.
Department of Ophthalmology, Tianjin Eye Hospital, Nankai University Affiliated Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Eye Institute, Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin, PR China.
Curr Eye Res. 2022 Sep;47(9):1329-1338. doi: 10.1080/02713683.2022.2072897. Epub 2022 Jun 30.
The development and recovery (REC) of myopia is associated with changing of choroidal thickness (CT) in the model of guinea pigs. Nitric oxide synthase (NOS) is an enzyme which can affect choroidal vasodilatation. This study wants to investigate the changes of choroidal vessel diameter (CVD) and NOS during the REC of form-deprivation (FD) myopia in guinea pigs.
Forty-eight guinea pigs were randomly assigned to the normal control (NC) group, FD group (FD for 21 d), and four REC groups: REC1/2 group (removal the deprivation and re-exposure to the normal environment for 1/2 d), REC1 group (1 d), REC 2 group (2 d), and REC7 group (7 d). CT was measured by optical coherence tomography (OCT), and CVD of foveal choroid was quantitatively assessed on OCT angiography images using MATLAB software at each time point. NOS in choroid was measured using enzyme-linked immunosorbent assays. Measurements were compared between groups and correlations between CT, CVD, and NOS were assessed using regression analyses.
CVD and CT in FD group were significantly smaller than in NC group (both < .05), while the NOS significantly larger ( < .001). When deprivation was removed, CVD and NOS were significantly larger and reached a peak in the REC1 group, while CT reached the peak in the REC2 group, then all gradually decreased, and no significant differences were observed in NC and REC7 group (all > .05). In the REC and NC groups, there was a significant positive correlation between CVD and NOS ( < .001), CVD and CT ( = .0092), but no correlation was found between NOS and CT ( > .05).
This study indicated that the CVD in guinea pigs could be significantly dilated following myopia REC, and this change coincides with changes in NOS and CT.
豚鼠模型中脉络膜厚度（CT）的变化与近视的发展和恢复（REC）有关。一氧化氮合酶（NOS）是一种能影响脉络膜血管扩张的酶。本研究旨在探讨在豚鼠形觉剥夺（FD）性近视的 REC 过程中脉络膜血管直径（CVD）和 NOS 的变化。
48 只豚鼠随机分为正常对照组（NC）、FD 组（21d FD）和 4 个 REC 组：REC1/2 组（去除剥夺并重新暴露于正常环境 1/2d）、REC1 组（1d）、REC2 组（2d）和 REC7 组（7d）。使用光学相干断层扫描（OCT）测量 CT，使用 MATLAB 软件定量评估 OCT 血管造影图像上的中心凹脉络膜的 CVD。使用酶联免疫吸附试验（ELISA）测量脉络膜中的 NOS。在每个时间点比较组间差异，并使用回归分析评估 CT、CVD 和 NOS 之间的相关性。
FD 组的 CVD 和 CT 明显小于 NC 组（均 < .05），而 NOS 明显更大（ < .001）。当剥夺被去除时，CVD 和 NOS 明显增大，并在 REC1 组中达到峰值，而 CT 在 REC2 组中达到峰值，然后逐渐下降，在 NC 和 REC7 组中均无明显差异（均 > .05）。在 REC 和 NC 组中，CVD 与 NOS 之间存在显著正相关（ < .001），CVD 与 CT 之间存在显著正相关（ = .0092），但 NOS 与 CT 之间无相关性（ > .05）。
本研究表明，豚鼠近视 REC 后，CVD 可明显扩张，这一变化与 NOS 和 CT 的变化一致。
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