豚鼠眼诱导性近视的脉络膜变化。
Choroidal changes in lens-induced myopia in guinea pigs.
机构信息
Shandong University of Traditional Chinese Medicine, No. 16369#, Jingshi Road, Jinan 250014, PR China; Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, PR China.
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, PR China; Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, PR China.
出版信息
Microvasc Res. 2021 Nov;138:104213. doi: 10.1016/j.mvr.2021.104213. Epub 2021 Jun 24.
INTRODUCTION
This study aimed to determine the role of the choroid in lens-induced myopia (LIM) in guinea pigs.
METHODS
Guinea pigs were randomly divided into two groups: a normal control (NC) group and a LIM group. Refraction and axial length (AL) were measured by streak retinoscopy and A-scan ultrasonography. The choroidal thickness (ChT), vessel density of the choriocapillaris (VDCC) and vessel density of the choroidal layer (VDCL) were assessed by Spectral-domain Optical Coherence Tomography Angiography (SD-OCT). In addition, the choroidal expression of nitric oxide synthase (NOS) enzymes at the mRNA and protein levels was analyzed by real-time fluorescence quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry.
RESULTS
In the LIM group, refraction and AL were increased significantly compared with those in the NC group at 2 weeks (refraction: LIM vs. NC, -4.23 ± 0.43 D vs. 2.20 ± 0.48 D; AL: LIM vs. NC, 8.36 ± 0.05 mm vs. 8.22 ± 0.03 mm) and 4 weeks (refraction: LIM vs. NC, -5.88 ± 0.49 D vs. 1.63 ± 0.41 D; AL: 8.57 ± 0.06 mm vs. 8.40 ± 0.04 mm). The ChT and VDCC were decreased significantly compared with those in the NC group at 2 weeks (ChT: LIM vs. NC, 60.92 ± 8.15 μm vs. 79.11 ± 7.47 μm; VDCC: LIM vs. NC, 23.43 ± 3.85% vs. 28.74 ± 4.11%) and 4 weeks (ChT: LIM vs. NC, 48.43 ± 6.85 μm vs. 76.38 ± 7.84 μm; VDCC: LIM vs. NC, 21.29 ± 2.17% vs. 27.64 ± 2.91%). The VDCL was also decreased compared with that in the NC group at 2 weeks and 4 weeks (NC vs. LIM, 24.87 ± 5.16% vs. 22.45 ± 3.26%; 23.37 ± 5.85% vs. 21.39 ± 2.62%; all P > 0.05). Moreover, the ChT was positively correlated with the VDCC and VDCL. The mRNA and protein expression of NOS enzymes (eNOS and nNOS) was increased.
CONCLUSIONS
During the development of myopia, the ChT, VDCC and VDCL were decreased, while NOS expression in the choroid was increased. The expression of NOS was negatively correlated with the ChT, VDCC and VDCL. NO may play an important role in regulating the choroid during myopia development.
简介
本研究旨在探讨脉络膜在豚鼠眼诱导性近视（LIM）中的作用。
方法
豚鼠随机分为正常对照组（NC）和 LIM 组。采用条纹视网膜镜和 A 型超声扫描测量屈光度和眼轴（AL）。采用谱域光相干断层扫描血管造影（SD-OCT）评估脉络膜厚度（ChT）、脉络膜毛细血管血管密度（VDCC）和脉络膜层血管密度（VDCL）。此外，采用实时荧光定量 PCR、酶联免疫吸附试验（ELISA）和免疫组织化学法分析脉络膜中一氧化氮合酶（NOS）酶的 mRNA 和蛋白表达。
结果
在 LIM 组，与 NC 组相比，2 周（屈光度：LIM 比 NC，-4.23±0.43 D 比 2.20±0.48 D；AL：LIM 比 NC，8.36±0.05mm 比 8.22±0.03mm）和 4 周（屈光度：LIM 比 NC，-5.88±0.49 D 比 1.63±0.41 D；AL：8.57±0.06mm 比 8.40±0.04mm）时，屈光度和 AL 显著增加。与 NC 组相比，2 周（ChT：LIM 比 NC，60.92±8.15μm 比 79.11±7.47μm；VDCC：LIM 比 NC，23.43±3.85% 比 28.74±4.11%）和 4 周（ChT：LIM 比 NC，48.43±6.85μm 比 76.38±7.84μm；VDCC：LIM 比 NC，21.29±2.17% 比 27.64±2.91%）时，ChT 和 VDCC 显著降低。与 NC 组相比，2 周和 4 周时 VDCL 也降低（NC 比 LIM，24.87±5.16% 比 22.45±3.26%；23.37±5.85% 比 21.39±2.62%；均 P>0.05）。此外，ChT 与 VDCC 和 VDCL 呈正相关。NOS 酶（eNOS 和 nNOS）的 mRNA 和蛋白表达增加。
结论
在近视发展过程中，脉络膜厚度、VDCC 和 VDCL 降低，而脉络膜中 NOS 的表达增加。NOS 的表达与 ChT、VDCC 和 VDCL 呈负相关。NO 可能在近视发展过程中调节脉络膜中起重要作用。
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