Department of Immunology, National School of Biological Sciences, National Polytechnic Institute, Mexico City, México.
Laboratory of Tuberculosis, National Institute of Forestry, Agricultural and Livestock Research, Mexico City, México.
Int J Mycobacteriol. 2022 Apr-Jun;11(2):190-198. doi: 10.4103/ijmy.ijmy_71_22.
Bovine tuberculosis (bTB) is still a prominent threat to animal health; lacking an efficient vaccine, other than BCG to get rid of tuberculosis, the most effective way for this is culling and slaughtering the infected animals. There are several cellular, serological, and molecular tests for the diagnosis of the disease but the most practical one at the field level is the double skin testing with bovine and aviary tuberculins. This is not a very specific test but is sensitive enough to identify most diseased animals; adjunct practical tests are desirable to strengthen the utility of skin tests. All lymphoid and myeloid cells participate, in diverse grades, in the immune response to tuberculosis with neutrophils playing an unintended pathologic role. The study aimed to investigate the response of neutrophils to agents present in the sera of tuberculous cows.
We have developed a neutrophil-based test (N BT) to identify diseased cows within a herd suspected of having tuberculosis; a positive N BT correlates with a positive double skin test. In this test, healthy neutrophils are incubated with the sera of healthy or tuberculous cows for 3 and 6 h, and the nuclear morphologic changes are recorded and analyzed.
Sera from tuberculous but not from healthy cows induce nuclear alterations including pyknosis, swelling, apoptosis, and sometimes NETosis, in healthy neutrophils, and CFP 10 and ESAT 6 participate in the phenomenon.
We propose the N BT as an auxiliary tool for substantiating the diagnosis of bTB reinforcing the PPD test outcome to help decide whether or not a cow should be sacrificed.
牛结核病（bTB）仍然是动物健康的主要威胁；除了卡介苗（BCG）外，缺乏有效的疫苗来消除结核病，最有效的方法是淘汰和屠宰受感染的动物。有几种细胞、血清学和分子检测方法可用于诊断该疾病，但在现场最实用的方法是使用牛结核菌素和禽结核菌素进行双皮检测。这不是一个非常特异的检测方法，但足够敏感，可以识别大多数患病动物；需要辅助实用检测来增强皮试的效用。所有淋巴样和髓样细胞都以不同的程度参与对结核病的免疫反应，中性粒细胞起着意想不到的病理性作用。本研究旨在研究中性粒细胞对来自结核牛血清中存在的物质的反应。
我们开发了一种基于中性粒细胞的检测方法（NBT），用于识别疑似患有结核病的牛群中患病的奶牛；阳性的 NBT 与阳性双皮检测相关。在该检测中，健康的中性粒细胞与健康或结核奶牛的血清孵育 3 和 6 小时，记录和分析核形态变化。
来自结核牛但不是健康牛的血清诱导健康中性粒细胞发生核改变，包括固缩、肿胀、凋亡，有时还发生 NETosis，CFP 10 和 ESAT 6 参与了这一现象。
我们提出了 NBT 作为辅助工具，用于支持 bTB 的诊断，增强 PPD 检测结果，以帮助决定是否应该屠宰奶牛。
