Department of Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
Department of Nursing, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
J Spinal Cord Med. 2023 Sep;46(5):848-858. doi: 10.1080/10790268.2021.2024029. Epub 2022 Jul 1.
Incomplete spinal cord injury (SCI) is the most common spinal cord injury in clinic, however its mechanism is still not fully understood.
We constructed the rabbit spinal cord hemisection (SCH) model and used RT-PCR, western blotting, immunohistochemistry, and immunofluorescence experiments to explore the potential mechanism of SCI.
The sham operation (SH) group, the observation (OB, which is the SCH) group, the OB+ substance p (SP) inhibitor group, the OB + NK1R inhibitor group, the OB + NK1R agonist group and the OB + SP inhibitor + NK1R agonist group.
New Zealand white rabbits.
Use NK1R inhibitors, NK1R agonists, SP inhibitors to treat the SCH model.
IL-1β, IKKγ, IL-6 and NF-κB.
The results showed that nissl bodies, inflammatory cells and SP increased notably in the spinal cord cells of the rabbit SCH model. Through experiments with SP or NK1R inhibitors or NK1R agonists, we found that inhibiting SP/NK1R signaling can help improve SCH by inhibiting the release of pro-inflammatory cytokines IL-1β, IKKγ, IL-6 and NF-κB.
Animal experiments were approved by Ruijin Hospital, Shanghai Jiaotong University School of Medicine.
不完全性脊髓损伤（SCI）是临床上最常见的脊髓损伤，但其机制尚未完全阐明。
我们构建了兔脊髓半切（SCH）模型，并通过 RT-PCR、western blot、免疫组化和免疫荧光实验来探讨 SCI 的潜在机制。
假手术（SH）组、观察（OB，即 SCH）组、OB+P 物质（SP）抑制剂组、OB+NK1R 抑制剂组、OB+NK1R 激动剂组和 OB+SP 抑制剂+NK1R 激动剂组。
新西兰白兔。
用 NK1R 抑制剂、NK1R 激动剂、SP 抑制剂治疗 SCH 模型。
IL-1β、IKKγ、IL-6 和 NF-κB。
结果显示，兔 SCH 模型脊髓细胞中尼氏小体、炎性细胞和 SP 明显增加。通过 SP 或 NK1R 抑制剂或 NK1R 激动剂的实验，我们发现抑制 SP/NK1R 信号通路可以通过抑制促炎细胞因子 IL-1β、IKKγ、IL-6 和 NF-κB 的释放来帮助改善 SCH。
动物实验得到了上海交通大学医学院瑞金医院的批准。
Zotero 插件
