Gao Xiao-Wei, Zhang Xiao-Min, Liu Hai-Yan, Wang Shan-Shan, Dong Hua-Jiang
Tianjin Medical University Eye Institute and Tianjin Medical University School of Optometry and Ophthalmology, Tianjin Medical University Eye Hospital, Tianjin, 300384, China.
Department of Otolaryngology-Head and Neck Surgery, Second Hospital of Tianjin Medical University, Tianjin, 300211, China.
Int Ophthalmol. 2019 Oct;39(10):2283-2294. doi: 10.1007/s10792-018-01066-5. Epub 2019 Jan 3.
The mechanisms of naso-ocular interaction in allergic rhinoconjunctivitis are not well understood. Neurogenic inflammation affects both eyes and nose via the same neurogenic factors. The purpose of this study was to investigate the effects of neurogenic inflammation on conjunctival inflammation following nasal allergen provocation.
Sensitized rats were exposed to ovalbumin (OVA) via the nose. Parts of the nasal mucosa and conjunctivae were sliced and used for hematoxylin-eosin staining, immunohistochemical analysis, western blotting, and real-time polymerase chain reaction. The slides were observed under a light microscope, and the acquired images were analyzed. The levels of substance P (SP), vasoactive intestinal peptide (VIP), and nerve growth factor (NGF) were detected.
The levels of SP, VIP, and NGF were increased in both nasal mucosa and conjunctivae 1 h and 24 h after OVA administration (p < 0.05). Higher levels of SP, VIP, and NGF expression were observed in the nasal mucosa and conjunctivae 24 h after OVA administration (p < 0.05). Following damage of the nasal sensory nerves by capsaicin, the protein and mRNA levels of SP, VIP, and NGF were reduced.
In conclusion, the increased levels of VIP, SP, and NGF might be responsible for the ocular reaction following nasal challenge with allergen in rats.
变应性鼻结膜炎中鼻眼相互作用的机制尚未完全明确。神经源性炎症通过相同的神经源性因子影响眼和鼻。本研究旨在探讨神经源性炎症对鼻内过敏原激发后结膜炎症的影响。
将致敏大鼠经鼻暴露于卵清蛋白（OVA）。取部分鼻黏膜和结膜切片，用于苏木精-伊红染色、免疫组织化学分析、蛋白质印迹法和实时聚合酶链反应。在光学显微镜下观察玻片，并对采集的图像进行分析。检测P物质（SP）、血管活性肠肽（VIP）和神经生长因子（NGF）的水平。
给予OVA后1小时和24小时，鼻黏膜和结膜中SP、VIP和NGF的水平均升高（p<0.05）。给予OVA后24小时，鼻黏膜和结膜中SP、VIP和NGF的表达水平更高（p<0.05）。辣椒素损伤鼻感觉神经后，SP、VIP和NGF的蛋白质和mRNA水平降低。
总之，VIP、SP和NGF水平升高可能是大鼠鼻内给予过敏原激发后眼部反应的原因。
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