Alipour Maedeh, Nasiri Nahid, Kazemi Fariba, Zare Farahnaz, Sharifzadeh Sedigheh
Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Nat Prod Res. 2023 Mar;37(6):985-989. doi: 10.1080/14786419.2022.2095378. Epub 2022 Jul 1.
Hydroxyurea (HU) is an anti-cancer drug that is used for the treatment of hemoglobinopathies as a inducer. However, its dose-dependent effects have hampered its clinical reliability. Resveratrol (RSV) is an antioxidant and γ inducer. The present study aimed to assess their combined effects on the gene expression and reactive oxygen species (ROS) level of K562 cells. The results indicated that the γ-globin gene expression was approximately two folds higher in the group treated with RSV 50 µM + HU 25 µM in comparison to HU 100 μM alone (*** < 0.001). However, there was an inverse relationship between the expression of γ-globin gene and HU concentration in the combined groups. Furthermore, the combinations of RSV and HU significantly reduced ROS levels compared to single drugs. Overall, the combination of these compounds was an appropriate strategy for increasing expression, reducing oxidant levels, and alleviating the adverse effects of HU.
羟基脲（HU）是一种抗癌药物，作为诱导剂用于治疗血红蛋白病。然而，其剂量依赖性效应阻碍了其临床可靠性。白藜芦醇（RSV）是一种抗氧化剂和γ诱导剂。本研究旨在评估它们对K562细胞的γ-珠蛋白基因表达和活性氧（ROS）水平的联合作用。结果表明，与单独使用100μM HU相比，用50μM RSV + 25μM HU处理的组中γ-珠蛋白基因表达高出约两倍（***<0.001）。然而，联合组中γ-珠蛋白基因的表达与HU浓度之间存在负相关关系。此外，与单一药物相比，RSV和HU的组合显著降低了ROS水平。总体而言，这些化合物的组合是增加γ-珠蛋白表达、降低氧化剂水平和减轻HU不良反应的合适策略。
