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在虚拟冷驯化后，测量果蝇幼虫冷诱发神经活性和耐寒性的方案。

Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation.

机构信息

Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.

Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.

出版信息

STAR Protoc. 2022 Sep 16;3(3):101510. doi: 10.1016/j.xpro.2022.101510. Epub 2022 Jun 29.

DOI:10.1016/j.xpro.2022.101510
PMID:35776643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9253850/
Abstract

Here, we outline protocols to study cold acclimation in Drosophila from a neurobiological perspective, starting with fictive cold acclimation using a custom-built optogenetics-housing apparatus we call the OptoBox. We also provide detailed steps for single-unit electrophysiological recordings from larval cold nociceptors and a high-throughput cold-tolerance assay. These protocols expand the toolkit for the study of insect cold acclimation and nociception. For complete details on the use and execution of this protocol, please refer to Himmel et al. (2021).

摘要

在这里，我们从神经生物学的角度概述了研究果蝇冷驯化的方案，首先使用我们称之为 OptoBox 的定制光遗传学饲养设备进行虚拟冷驯化。我们还提供了从幼虫冷伤害感受器进行单细胞电生理记录和高通量耐寒性测定的详细步骤。这些方案扩展了研究昆虫冷驯化和伤害感受的工具包。有关此方案使用和执行的完整详细信息，请参阅 Himmel 等人。（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/7e940a94c43c/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/a68df2c0b724/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/6e3111f0e18b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/161a6b8065a3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/45bc325df11e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/125be816b28c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/dece7860257f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/73438a71f7a4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/7e940a94c43c/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/a68df2c0b724/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/6e3111f0e18b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/161a6b8065a3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/45bc325df11e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/125be816b28c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/dece7860257f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/73438a71f7a4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fabb/9253850/7e940a94c43c/gr7.jpg

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Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation.在虚拟冷驯化后，测量果蝇幼虫冷诱发神经活性和耐寒性的方案。
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