Zhang Jinkai, Zhao Cancan, Sheng Ruilong, Lin Kaili, Wang Xudong, Zhang Shilei
Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai 200011, China.
Department of Orthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China.
ACS Appl Mater Interfaces. 2022 Jul 13;14(27):30571-30581. doi: 10.1021/acsami.2c06454. Epub 2022 Jul 1.
Constructing hierarchical hybrid structures is considered a facile method to improve the osseointegration of implants. Herein, a hierarchical micro-/submicro-/nanostructured surface feature of Ti6Al4V implants (3DAT group) was successfully constructed by combining the inherently formed three-dimensional (3D)-printed microscale topography, acid-etched sub-micropits, and anodized nanotubes. Compared with the classical SLA surface, the microscale topography and sub-micropits increased the three-dimensional space for the cell growth and mechanical stability of implants, while the modification of nanotubes dramatically improved the surface hydrophilicity, protein adsorption, and biomineralization. Most importantly, the 3DAT surface feature possessed excellent osteogenic performance and , with the involvement of semaphorin 7A (Sema7A) as revealed by RNA-seq through the ITGB1/FAK/ERK signaling pathway. The present study suggested that the hierarchically structured surface design strategy could accelerate the osseointegration rate of 3D-printed Ti6Al4V implants, promising personalized reconstruction of bone defects.
构建分层混合结构被认为是一种改善植入物骨整合的简便方法。在此，通过结合固有形成的三维（3D）打印微观形貌、酸蚀亚微坑和阳极氧化纳米管，成功构建了Ti6Al4V植入物的分层微/亚微/纳米结构表面特征（3DAT组）。与经典的SLA表面相比，微观形貌和亚微坑增加了细胞生长的三维空间以及植入物的机械稳定性，而纳米管的修饰显著改善了表面亲水性、蛋白质吸附和生物矿化。最重要的是，3DAT表面特征具有优异的成骨性能，并且通过RNA测序揭示，在信号素7A（Sema7A）的参与下，通过ITGB1/FAK/ERK信号通路发挥作用。本研究表明，分层结构的表面设计策略可以加速3D打印Ti6Al4V植入物的骨整合速率，有望实现骨缺损的个性化重建。
