Wang Jinjin, Wang Yazheng, Li Yi, He Yide, Song Wen, Wang Qintao, Zhang Yumei, He Chenyang
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shannxi Province 710032, China.
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shannxi Province 710032, China.
Regen Biomater. 2023 Feb 17;10:rbad012. doi: 10.1093/rb/rbad012. eCollection 2023.
The comprehensive recognition of communications between bone marrow mesenchymal stem cells (bm-MSCs) and macrophages in the peri-implant microenvironment is crucial for implantation prognosis. Our previous studies have clarified the indirect influence of Ti surface topography in the osteogenic differentiation of bm-MSCs through modulating macrophage polarization. However, cell communication is commutative and multi-directional. As the immune regulatory properties of MSCs have become increasingly prominent, whether bm-MSCs could also play an immunomodulatory role on macrophages under the influence of Ti surface topography is unclear. To further illuminate the communications between bm-MSCs and macrophages, the bm-MSCs inoculated on Ti with nanoporous topography were indirectly co-cultured with macrophages, and by blocking exosome secretion or extracting the purified exosomes to induce independently, we bidirectionally confirmed that under the influence of TiO nanoporous topography with 80-100 nm tube diameters, bm-MSCs can exert immunomodulatory effects through exosome-mediated paracrine actions and induce M1 polarization of macrophages, adversely affecting the osteogenic microenvironment around the implant. This finding provides a reference for the optimal design of the implant surface topography for inducing better bone regeneration.
全面认识骨髓间充质干细胞（bm-MSCs）与种植体周围微环境中巨噬细胞之间的相互作用对于种植预后至关重要。我们之前的研究已经阐明了钛表面形貌通过调节巨噬细胞极化对bm-MSCs成骨分化的间接影响。然而，细胞间通讯是相互且多向的。随着间充质干细胞免疫调节特性日益突出，在钛表面形貌影响下bm-MSCs是否也能对巨噬细胞发挥免疫调节作用尚不清楚。为进一步阐明bm-MSCs与巨噬细胞之间的相互作用，将接种于具有纳米多孔形貌钛表面的bm-MSCs与巨噬细胞间接共培养，通过阻断外泌体分泌或提取纯化外泌体进行单独诱导，我们双向证实了在管径为80 - 100 nm的TiO纳米多孔形貌影响下，bm-MSCs可通过外泌体介导的旁分泌作用发挥免疫调节作用并诱导巨噬细胞向M1极化，对种植体周围的成骨微环境产生不利影响。这一发现为诱导更好骨再生的种植体表面形貌优化设计提供了参考。
