Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kita gun, Kagawa, 761-0795, Japan.
Biotechnology Program, Faculty of Science, Galala University, Suze, Galala, 43511, Egypt; Botany Department, Faculty of Science, Aswan University, Aswan, 81528, Egypt.
Environ Pollut. 2022 Sep 1;308:119687. doi: 10.1016/j.envpol.2022.119687. Epub 2022 Jun 28.
To examine the potential role of acetate in conferring cadmium (Cd) stress tolerance in lentil (Lens culinaris), several phenotypical and physio-biochemical properties have been examined in Cd-stressed lentil seedlings following acetate applications. Acetate treatment inhibited the translocation of Cd from roots to shoots, which resulted in a minimal reduction in photosynthetic pigment contents. Additionally, acetate-treated lentil showed higher shoot (1.1 and 11.72%) and root (4.98 and 30.64%) dry weights compared with acetate-non-treated plants under low-Cd and high-Cd concentrations, respectively. Concurrently, acetate treatments increase osmoprotection under low-Cd stress through proline accumulation (24.69%), as well as enhancement of antioxidant defense by increasing ascorbic acid content (239.13%) and catalase activity (148.51%) under high-Cd stress. Acetate-induced antioxidant defense resulted in a significant diminution in hydrogen peroxide, malondialdehyde and electrolyte leakage in Cd-stressed lentil seedlings. Our results indicated that acetate application mitigated oxidative stress-induced damage by modulating antioxidant defense and osmoprotection, and reducing root-to-shoot Cd transport. These findings indicate an important contribution of acetate in mitigating the Cd toxicity during growth and development of lentil seedlings, and suggest that the exogenous applications of acetate could be an economical and new avenue for controlling heavy metal-caused damage in lentil, and potentially in many other crops.
为了研究乙酸在赋予菜豆（Lens culinaris）对镉（Cd）胁迫耐受性方面的潜在作用，在向 Cd 胁迫下的菜豆幼苗施加乙酸后，研究了几种表型和生理生化特性。乙酸处理抑制了 Cd 从根部向地上部的转运，从而使光合色素含量的减少最小化。此外，与未处理的乙酸的菜豆相比，在低 Cd 和高 Cd 浓度下，乙酸处理的菜豆地上部（1.1 和 11.72%）和根部（4.98 和 30.64%）的干重分别更高。同时，乙酸处理通过脯氨酸积累（24.69%）在低 Cd 胁迫下增加渗透保护，通过增加抗坏血酸含量（239.13%）和过氧化氢酶活性（148.51%）在高 Cd 胁迫下增强抗氧化防御，从而提高渗透保护。乙酸诱导的抗氧化防御导致 Cd 胁迫下菜豆幼苗中过氧化氢、丙二醛和电解质渗漏显著减少。我们的结果表明，乙酸通过调节抗氧化防御和渗透保护，减少根到茎的 Cd 转运，从而减轻氧化应激诱导的损伤。这些发现表明，在菜豆幼苗的生长和发育过程中，乙酸的应用对减轻 Cd 毒性具有重要贡献，并表明外源乙酸的应用可能是一种经济有效的新途径，可以控制重金属对菜豆及其他许多作物造成的损害。
