Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan.
Hyogo Prefectural Technology Center of Agriculture, Forestry and Fisheries, Kasai, Hyogo, 679-0198, Japan.
Sci Rep. 2022 Jul 1;12(1):11154. doi: 10.1038/s41598-022-15146-1.
In this study, using enteric methane emissions, we investigated the metabolic characteristics of Japanese Black cattle. Their methane emissions were measured at early (age 13 months), middle (20 months), and late fattening phases (28 months). Cattle with the highest and lowest methane emissions were selected based on the residual methane emission values, and their liver transcriptome, blood metabolites, hormones, and rumen fermentation characteristics were analyzed. Blood β-hydroxybutyric acid and insulin levels were high, whereas blood amino acid levels were low in cattle with high methane emissions. Further, propionate and butyrate levels differed depending on the enteric methane emissions. Hepatic genes, such as SERPINI2, SLC7A5, ATP6, and RRAD, which were related to amino acid transport and glucose metabolism, were upregulated or downregulated during the late fattening phase. The above mentioned metabolites and liver transcriptomes could be used to evaluate enteric methanogenesis in Japanese Black cattle.
本研究通过测定肠道甲烷排放，研究了日本黑牛的代谢特性。在育肥早期（13 月龄）、中期（20 月龄）和后期（28 月龄）分别对其进行了甲烷排放量的测量。根据残留甲烷排放量值选择甲烷排放量最高和最低的牛，分析其肝脏转录组、血液代谢物、激素和瘤胃发酵特性。高甲烷排放牛血液中 β-羟丁酸和胰岛素水平较高，而血液中氨基酸水平较低。此外，丙酸和丁酸水平因肠道甲烷排放而异。在育肥后期，与氨基酸转运和葡萄糖代谢相关的 SERPINI2、SLC7A5、ATP6 和 RRAD 等肝基因上调或下调。上述代谢物和肝脏转录组可用于评估日本黑牛肠道甲烷生成。
