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Cacna2d2 抑制颈椎脊髓病大鼠模型手术减压后轴突再生。

Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy.

机构信息

Department of Spinal Surgery, Yantaishan Hospital, No. 10087, Keji Avenue, Laishan District, 264000, Yantai, China.

Basic Department, Yantai Vocational College, 264000, Yantai, China.

出版信息

BMC Neurosci. 2022 Jul 1;23(1):42. doi: 10.1186/s12868-022-00727-7.

DOI:10.1186/s12868-022-00727-7
PMID:35778700
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9248146/
Abstract

BACKGROUND

Cervical spondylotic myelopathy (CSM) is a clinically symptomatic condition due to spinal cord compression, leading to spinal cord dysfunction. Surgical decompression is the main treatment of CSM, but the mechanisms of axonal regeneration after surgical decompression are still fragmentary.

METHODS

In a rat model of CSM, the cacna2d2 (α2δ2) expression levels in anterior horn of spinal cord were observed following compression and decompression by western blot and immunofluorescence. The expression levels of 5 hydroxytryptamine (5HT) and GAP43 were also analyzed by immunofluorescence. Furthermore, gabapentin intervention was performed for 4 weeks after decompression to analyze the changes of behaviors and anterior horn of spinal cords.

RESULTS

Following decompression, the expression levels of α2δ2 in the anterior horn of spinal cord were decreased, but the expression levels of 5HT andGAP43 were increased. Compared with the vehicle treated rats, gabapentin treatment for 4 weeks ameliorated the behaviors of rats and improved the damaged anterior horn of spinal cord. Besides, inhibition of α2δ2 through gabapentin intervention enhanced the axonal regeneration in the anterior horn of damaged spinal cord.

CONCLUSIONS

Inhibition of α2δ2 could enhance axonal recovery in anterior horn of damaged spinal cord induced by CSM after surgical decompression, providing a potential method for promoting axon regeneration following surgery.

摘要

背景

颈椎脊髓病（CSM）是一种由于脊髓受压导致脊髓功能障碍的临床症状。手术减压是 CSM 的主要治疗方法，但手术减压后轴突再生的机制仍不完整。

方法

通过 Western blot 和免疫荧光观察大鼠 CSM 模型中脊髓前角 cacna2d2（α2δ2）的表达水平在受压和解压后的变化。通过免疫荧光还分析了 5 羟色胺（5HT）和 GAP43 的表达水平。此外，在减压后进行加巴喷丁干预 4 周，分析行为和脊髓前角的变化。

结果

减压后，脊髓前角 α2δ2 的表达水平降低，但 5HT 和 GAP43 的表达水平升高。与 vehicle 处理的大鼠相比，加巴喷丁治疗 4 周可改善大鼠的行为并改善受损的脊髓前角。此外，通过加巴喷丁干预抑制 α2δ2 可增强受损脊髓前角中的轴突再生。

结论

抑制 α2δ2 可能会增强 CSM 手术后减压引起的受损脊髓前角中的轴突恢复，为手术后促进轴突再生提供了一种潜在的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/e68de80be524/12868_2022_727_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/cdf7e8c89891/12868_2022_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/ea76dd95781f/12868_2022_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/e70105d5ec27/12868_2022_727_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/4bb523ccbdf0/12868_2022_727_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/ddb20eb051db/12868_2022_727_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/f9aaaaaa52e6/12868_2022_727_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/e68de80be524/12868_2022_727_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/cdf7e8c89891/12868_2022_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/ea76dd95781f/12868_2022_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/e70105d5ec27/12868_2022_727_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/4bb523ccbdf0/12868_2022_727_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/ddb20eb051db/12868_2022_727_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/f9aaaaaa52e6/12868_2022_727_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9489/9248146/e68de80be524/12868_2022_727_Fig7_HTML.jpg

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