Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Provincial Engineering Laboratory of Insects Bio-reactor, Nanyang Normal University, Nanyang, PR China.
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, PR China.
BMC Vet Res. 2022 Jul 1;18(1):254. doi: 10.1186/s12917-022-03361-x.
In addition to Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum, a few hemoplasma species that mainly infect other livestock have been detected in dogs. 'Candidatus Mycoplasma haemobos' (Ca. M. haemobos) has been found in a variety of animals in China. The present study was aimed to investigate the occurrence of 'Ca. M. haemobos' infections in dogs and ticks collected from the Henan province, China.
Overall, 55 dog blood samples and 378 ticks on skins were collected from anemic and healthy dogs, and these samples were subjected to PCR, sequence analysis, and identification. The results showed that Haemaphysalis longicornis (266) and Rhipicephalus (Boophilus) microplus (112) were the only two parasitic ticks on dogs. Molecular detection revealed that 163 M. haemocanis, 88 'Ca. M. haemobos' and 32 Anaplasma platys positive amplicons could be amplified from dogs, H. longicornis and R. (B.) microplus. In addition, co-infections (M. haemocanis + A. platys and 'Ca. M. haemobos'+ A. platys) could be also detected.
To the best of our knowledge, this is the first molecular evidence of 'Ca. M. haemobos' natural infection in dogs and tick species identified as H. longicornis and R. (B.) microplus from China.
除犬嗜血支原体和犬血红支原体外，在犬中还检测到一些主要感染其他家畜的血原体种。在中国的多种动物中发现了“候选血支原体”（Ca. M. haemobos）。本研究旨在调查中国河南省犬和蜱中“候选血支原体”感染的发生情况。
从贫血和健康的犬中采集了 55 份犬血样和 378 份皮肤蜱样，并对这些样本进行了 PCR、序列分析和鉴定。结果表明，只有长角血蜱（266 只）和微小牛蜱（112 只）是寄生在犬上的两种蜱。分子检测显示，从犬、长角血蜱和微小牛蜱中可扩增出 163 个犬嗜血支原体、88 个“候选血支原体”和 32 个无形体阳性扩增子。此外，还可检测到混合感染（犬嗜血支原体+无形体和“候选血支原体”+无形体）。
据我们所知，这是首次在中国从犬和鉴定为长角血蜱和微小牛蜱的蜱中发现“候选血支原体”自然感染的分子证据。
